Universidade Federal do Rio Grande, Programa de Pós-Graduação em Oceanografia Biológica, Av. Itália km 8, 96201-900 Rio Grande, Rio Grande do Sul, Brazil.
Aquat Toxicol. 2013 Aug 15;138-139:60-9. doi: 10.1016/j.aquatox.2013.04.009. Epub 2013 Apr 29.
The responses of a large suite of biochemical and genetic parameters were evaluated in tissues (liver, gills, muscle and erythrocytes) of the estuarine guppy Poecilia vivipara exposed to waterborne copper in salt water (salinity 24 ppt). Activities of antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, and glutathione S-transferase), metallothionein-like protein concentration, reactive oxygen species (ROS) content, antioxidant capacity against peroxyl radicals (ACAP), and lipid peroxidation (LPO) were evaluated in liver, gills, and muscle. Comet assay score and nuclear abnormalities and micronucleated cell frequency were analyzed in peripheral erythrocytes. The responses of these parameters were evaluated in fish exposed (96 h) to environmentally relevant copper concentrations (5, 9 and 20 μg L⁻¹). In control and copper-exposed fish, no mortality was observed over the experimental period. Almost all biochemical and genetic parameters proved to be affected by waterborne copper exposure. However, the response of catalase activity in liver, ROS, ACAP and LPO in muscle, gills and liver, and DNA damages in erythrocytes clearly showed to be dependent on copper concentration in salt water. Therefore, the use of these parameters could be of relevance in the scope of biomonitoring programs in salt water environments contaminated with copper.
研究了盐水中（盐度 24 ppt）暴露于铜的河口孔雀鱼（Poecilia vivipara）组织（肝脏、鳃、肌肉和红细胞）中大量生化和遗传参数的反应。评估了抗氧化酶（超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶和谷胱甘肽 S-转移酶）、金属硫蛋白样蛋白浓度、活性氧（ROS）含量、过氧自由基抗氧化能力（ACAP）和脂质过氧化（LPO）在肝脏、鳃和肌肉中的活性。外周红细胞中的彗星试验评分和核异常以及微核细胞频率进行了分析。评估了这些参数在暴露于环境相关铜浓度（5、9 和 20 μg/L）下的鱼中的反应。在实验期间，对照组和铜暴露组的鱼均未死亡。几乎所有的生化和遗传参数都被证明受到水铜暴露的影响。然而，肝脏中过氧化氢酶活性、肌肉、鳃和肝脏中的 ROS、ACAP 和 LPO 以及红细胞中的 DNA 损伤的反应显然依赖于盐水中的铜浓度。因此，这些参数的使用可能与受铜污染的盐水环境中的生物监测计划有关。
