Pôle odontologie, CHU Nice, Nice, France; Faculté d'odontologie, Université de Nice Sophia-Antipolis, Nice, France; Equipe de Microbiologie, EA 1254, Université de Rennes I, Rennes, France.
Lett Appl Microbiol. 2013 Oct;57(4):303-9. doi: 10.1111/lam.12112. Epub 2013 Jun 20.
In oral microbiome, because of the abundance of commensal competitive flora, selective media with antibiotics are necessary for the recovery of fastidious Capnocytophaga species. The performances of six culture media (blood agar, chocolate blood agar, VCAT medium, CAPE medium, bacitracin chocolate blood agar and VK medium) were compared with literature data concerning five other media (FAA, LB, TSBV, CapR and TBBP media). To understand variable growth on selective media, the MICs of each antimicrobial agent contained in this different media (colistin, kanamycin, trimethoprim, trimethoprim-sulfamethoxazole, vancomycin, aztreonam and bacitracin) were determined for all Capnocytophaga species. Overall, VCAT medium (Columbia, 10% cooked horse blood, polyvitaminic supplement, 3·75 mg l(-1) of colistin, 1·5 mg l(-1) of trimethoprim, 1 mg l(-1) of vancomycin and 0·5 mg l(-1) of amphotericin B, Oxoid, France) was the more efficient selective medium, with regard to the detection of Capnocytophaga species from oral samples (P < 0·001) and the elimination of commensal clinical species (P < 0·001). The demonstrated superiority of VCAT medium, related to its antibiotic content, made its use indispensable for the optimal isolation of Capnocytophaga species from polymicrobial samples.
Isolation of Capnocytophaga species is important for the proper diagnosis and treatment of the systemic infections they cause and for epidemiological studies of periodontal flora. We showed that in pure culture, a simple blood agar allowed the growth of all Capnocytophaga species. Nonetheless, in oral samples, because of the abundance of commensal competitive flora, selective media with antibiotics are necessary for the recovery of Capnocytophaga species. The demonstrated superiority of VCAT medium made its use essential for the optimal detection of this bacterial genus. This work showed that extreme caution should be exercised when reporting the isolation of Capnocytophaga species from oral polymicrobial samples, because the culture medium is a determining factor.
在口腔微生物组中，由于共生竞争菌群的丰富度，选择性培养基中需要添加抗生素以恢复苛养的噬二氧化碳噬纤维菌属物种。本文比较了六种培养基（血琼脂、巧克力血琼脂、VCAT 培养基、CAPE 培养基、杆菌肽巧克力血琼脂和 VK 培养基）与文献中其他五种培养基（FAA、LB、TSBV、CapR 和 TBBP 培养基）的性能。为了了解选择性培养基上可变的生长情况，本文测定了这些不同培养基中（多粘菌素、卡那霉素、甲氧苄啶、甲氧苄啶-磺胺甲恶唑、万古霉素、氨曲南和杆菌肽）每种抗菌药物的 MIC 值。总的来说，VCAT 培养基（哥伦比亚琼脂、10%熟马血、多种维生素补充剂、3.75mg/L 多粘菌素、1.5mg/L 甲氧苄啶、1mg/L 万古霉素和 0.5mg/L 两性霉素 B，Oxoid，法国）是检测口腔样本中噬二氧化碳噬纤维菌属物种最有效的选择性培养基（P<0.001），并能消除共生临床物种（P<0.001）。VCAT 培养基的抗生素含量使其具有优越性，对于从混合微生物样本中最佳分离噬二氧化碳噬纤维菌属物种来说是不可或缺的。
分离噬二氧化碳噬纤维菌属物种对于正确诊断和治疗它们引起的全身感染以及进行牙周菌群的流行病学研究非常重要。我们表明，在纯培养物中，简单的血琼脂可使所有噬二氧化碳噬纤维菌属物种生长。尽管如此，在口腔样本中，由于共生竞争菌群的丰富度，选择性培养基中需要添加抗生素以恢复噬二氧化碳噬纤维菌属物种。VCAT 培养基的优越性证明了其对于最佳检测这种细菌属的必要性。这项工作表明，在报告从口腔混合微生物样本中分离噬二氧化碳噬纤维菌属物种时应格外小心，因为培养基是一个决定性因素。
