Laboratorio de Biotecnología Bacteriana, Instituto de Ciencia y Tecnología de Alimentos y Nutrición, ICTAN-CSIC, Madrid, Spain.
Meat Sci. 2013 Oct;95(2):272-80. doi: 10.1016/j.meatsci.2013.05.019. Epub 2013 May 18.
Technological and safety-related properties were analyzed in lactic acid bacteria isolated from Spanish dry-cured sausages in order to select them as starter cultures. In relation to technological properties, all the strains showed significative nitrate reductase activity; Lactobacillus plantarum, Lactobacillus paracasei and 52% of the Enterococcus faecium strains showed lipolytic activity and only Lactobacillus sakei strains (43%) were able to form biofilms. Related to safety aspects, E. faecium strains were the most resistant to antibiotics, whereas, L. sakei strains were the most sensitive. In relation to virulence factors, in the E. faecium strains analyzed, only the presence of efaA gene was detected. The analysis of biogenic amine production showed that most E. faecium strains and L. sakei Al-142 produced tyramine. In conclusion, L. paracasei Al-128 and L. sakei Al-143 strains possess the best properties to be selected as adequate and safe meat starter cultures.
为了选择合适的干腌香肠发酵剂,本研究分析了西班牙干腌香肠中分离出的乳酸菌的技术和安全相关特性。就技术特性而言,所有菌株均表现出显著的硝酸盐还原酶活性;植物乳杆菌、副干酪乳杆菌和 52%的粪肠球菌菌株具有脂肪酶活性,只有清酒乳杆菌菌株(43%)能够形成生物膜。就安全性方面而言,粪肠球菌菌株对抗生素的耐药性最强,而清酒乳杆菌菌株的敏感性最强。就毒力因子而言,在所分析的粪肠球菌菌株中,仅检测到efaA 基因的存在。生物胺产生分析表明,大多数粪肠球菌菌株和清酒乳杆菌 Al-142 产生酪胺。综上所述,副干酪乳杆菌 Al-128 和清酒乳杆菌 Al-143 菌株具有作为合适且安全的肉类发酵剂的最佳特性。