College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
Int J Biol Macromol. 2013 Sep;60:206-12. doi: 10.1016/j.ijbiomac.2013.05.030. Epub 2013 Jun 5.
In vitro, the effects of astragalus polysaccharide liposome (APSL) on splenocyte proliferation of mice were determined. The results showed that APSL could significantly promote splenocyte proliferation synergistically with PHA and LPS and the efficacy were superior to those of astragalus polysaccharide (APS) and blank liposome (BL). In immune response experiment, the adjuvant effect of APSL at three doses, APS, BL and aluminum hydroxide (alum) were compared on mice immunized subcutaneously with ovalbumin (OVA). The results showed that APSL could significantly promote splenocyte proliferation, enhance specific IgG, IgG1 and IgG2a antibody responses, promote IFN-γ and IL-6 secretion, and the efficacy were significantly better than alum at most time points. These results indicated that APSL could significantly improve the adjuvanticity and drug action of APS, and its high and medium doses possessed the best efficacy. Therefore, the liposome would be expected to exploit into a new-type preparation of APS.
在体外，测定了黄芪多糖脂质体（APSL）对小鼠脾细胞增殖的影响。结果表明，APSL 可与 PHA 和 LPS 协同显著促进脾细胞增殖，其疗效优于黄芪多糖（APS）和空白脂质体（BL）。在免疫反应实验中，比较了 APSL 在三个剂量、APS、BL 和氢氧化铝（alum）在皮下免疫卵清蛋白（OVA）的小鼠中的佐剂作用。结果表明，APSL 可显著促进脾细胞增殖，增强特异性 IgG、IgG1 和 IgG2a 抗体反应，促进 IFN-γ 和 IL-6 的分泌，其疗效在大多数时间点均明显优于 alum。这些结果表明，APSL 可显著提高 APS 的佐剂作用和药物作用，其高剂量和中剂量具有最佳疗效。因此，脂质体有望开发成为 APS 的新型制剂。
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