National Reference Laboratory of Veterinary Drug Residues (SCAU), College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
Analyst. 2013 Aug 21;138(16):4579-84. doi: 10.1039/c3an36806h. Epub 2013 Jun 10.
In the determination of nine β-agonists including salbutamol, terbutaline, cimaterol, fenoterol, clorprenaline, ractopamine, tulobuterol, clenbuterol and penbuterol in porcine, bovine, lamb and chicken muscle, liver and urine samples with LC-MS/MS, calibration curves prepared in solvent (SC) were compared with those prepared in each matrix (MC) for all analytes. Significant differences (P < 0.05) between SC and each MC for most analytes indicated the existence of matrix effects and the necessity of using MC for quantitation to compensate MEs. Then MC in each muscle, liver and urine sample was compared with the select potential representative matrix, followed by validating the recoveries of nine analytes calculated through the MC in the potential representative matrix and MC in their corresponding matrices, respectively. The results suggested that porcine muscle could be selected as a representative matrix to calibrate β-agonist residues in bovine, lamb and chicken muscle samples.
采用 LC-MS/MS 法测定猪、牛、羊和鸡肌肉、肝脏和尿液样品中的 9 种β-激动剂（包括沙丁胺醇、特布他林、西马特罗、非诺特罗、氯丙那林、莱克多巴胺、妥布特罗、克伦特罗和喷布特罗）时，比较了溶剂（SC）和各基质（MC）中配制的校准曲线。对于大多数分析物，SC 与各 MC 之间存在显著差异（P < 0.05），表明存在基质效应，需要使用 MC 进行定量以补偿 ME。然后，比较了各肌肉、肝脏和尿液样品中的 MC 与选择的潜在代表性基质，随后验证了通过潜在代表性基质中的 MC 和相应基质中的 MC 计算的 9 种分析物回收率。结果表明，猪肌肉可作为代表性基质，用于校准牛、羊和鸡肌肉样品中的β-激动剂残留。
