Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Skin Pharmacol Physiol. 2013;26(3):160-74. doi: 10.1159/000351341. Epub 2013 Jun 6.
Microdialysis is a method that has been used for decades to recover endogenous mediators, metabolites and drugs from the interstitial space in several tissues of both animals and humans. The principle of microdialysis is the flux of compounds across a semipermeable membrane. The application of microdialysis as a method of drug delivery is a process referred to as retrodialysis, i.e. the introduction of a substance into the extracellular space via a microdialysis probe. Thus, microdialysis also offers opportunities to deliver mediators and drugs to target tissues by adding solutes to the perfusion medium. In this context, retrodialysis combines a method for minimally invasive delivery with a sampling method to study biological processes in health and disease. The aim of this review is to give insight into the use of retrodialysis by outlining examples of retrodialysis studies focusing on applications in skin in animal studies, human experimental investigations and clinical settings.
微透析是一种几十年来一直被用于从动物和人体的几种组织的细胞外间隙中回收内源性介质、代谢物和药物的方法。微透析的原理是化合物通过半透膜的流量。将微透析作为药物输送方法的应用过程称为逆行透析，即通过微透析探针将物质引入细胞外空间。因此，通过向灌流液中添加溶质，微透析还为将介质和药物递送至靶组织提供了机会。在这种情况下，逆行透析将微创输送方法与采样方法相结合，以研究健康和疾病中的生物学过程。本文综述的目的是通过举例说明逆行透析研究，重点介绍动物研究、人体实验研究和临床环境中的皮肤应用，从而深入了解逆行透析的应用。
