Reuss S, Hurlbut E C, Speh J C, Moore R Y
Department of Neurology, School of Medicine, State University of New York, Stony Brook 11794.
Am J Anat. 1990 Jun;188(2):163-74. doi: 10.1002/aja.1001880206.
The distribution of neuropeptide Y-immunoreactive (NPY-IR) perikarya, fibers, and terminals was investigated in the brain of two species of hibernatory ground squirrels, Spermophilus tridecemlineatus and S. richardsonii, by means of immunohistochemistry. In the telencephalic and diencephalic structures studied, distinct patterns of NPY-IR were observed which were essentially identical in male and female animals of both species. No differences in amount or distribution of NPY-IR structures were observed between animals which had been in induced hibernation for several months before sacrifice in March/April and those sacrificed one week after their capture in May. In some brain structures (e.g., the hypothalamic arcuate nucleus), IR cell bodies were observed only after pretreatment with colchicine. NPY-IR perikarya and fibers were found in the cerebral cortex, caudate nucleus-putamen, and dorsal part of the lateral septal nucleus. Dense fiber plexuses were seen in the lateral and medial parts of the bed nucleus of the stria terminalis. The numbers of IR perikarya observed in the medial part of the nucleus increased following intraventricular colchicine injections. The accumbens nucleus exhibited few IR cells and many fibers. Claustrum and endopiriform nuclei showed a considerable number of stained cells and fibers that increased in number and staining intensity in colchicine-treated ground squirrels. The induseum griseum showed a small band of IR cell bodies and varicose fibers. Bipolar of multipolar IR cells and varicose fibers were found in the basal nucleus of the amygdala. Dense fiber plexuses as well as IR terminals were seen in the median, medial, and lateral preoptic areas of the hypothalamus. Terminals and relatively few fibers were located in the periventricular, paraventricular, and supraoptic nuclei. The anterior, lateral, dorsomedial, and ventromedial hypothalamic nuclei contained relatively large numbers of terminals and fibers. In the suprachiasmatic nuclei, dense terminals were distributed mainly in the ventromedial subdivision. In the median eminence, immunoreactive terminals were concentrated in the external layer, with fibers predominant in the internal layer. NPY-IR perikarya were observed only in the arcuate nucleus of the hypothalamus and only following colchicine treatment. In the epithalamus (superficial part of the pineal gland and habenular nuclei), varicose fibers appeared mainly in perivascular locations (pineal) or as a dense plexus (habenular nuclei). These results from ground squirrels are discussed in comparison to those obtained in other species and with regard to considerations of the physiological role of NPY.
采用免疫组织化学方法，研究了两种冬眠地松鼠（三线松鼠和理查森松鼠）脑中神经肽Y免疫反应性（NPY-IR）神经元胞体、纤维和终末的分布情况。在所研究的端脑和间脑结构中，观察到明显的NPY-IR模式，这在两种松鼠的雄性和雌性动物中基本相同。在3月/4月处死前处于诱导冬眠状态数月的动物与5月捕获一周后处死的动物之间，未观察到NPY-IR结构的数量或分布差异。在一些脑结构（如下丘脑弓状核）中，仅在秋水仙碱预处理后才观察到免疫反应性细胞体。在大脑皮层、尾状核-壳核和外侧隔核背侧部分发现了NPY-IR神经元胞体和纤维。终纹床核的外侧和内侧部分可见密集的纤维丛。脑室内注射秋水仙碱后，核内侧部分观察到的免疫反应性神经元胞体数量增加。伏隔核中免疫反应性细胞较少，纤维较多。屏状核和内梨状核显示有大量染色细胞和纤维，在秋水仙碱处理的地松鼠中数量和染色强度增加。胼胝体显示有一小带免疫反应性细胞体和曲张纤维。杏仁核基底核中发现双极或多极免疫反应性细胞和曲张纤维。下丘脑的视前正中区、内侧区和外侧区可见密集的纤维丛以及免疫反应性终末。终末和相对较少的纤维位于室周核、室旁核和视上核。下丘脑前核、外侧核、背内侧核和腹内侧核含有相对大量的终末和纤维。在视交叉上核中，密集的终末主要分布在腹内侧亚区。在正中隆起中，免疫反应性终末集中在外层，纤维在内层占主导。仅在下丘脑弓状核中观察到NPY-IR神经元胞体，且仅在秋水仙碱处理后出现。在上丘脑（松果体浅层和缰核）中，曲张纤维主要出现在血管周围位置（松果体）或形成密集丛（缰核）。将地松鼠的这些结果与其他物种的结果进行了比较，并讨论了NPY的生理作用。
