甲基丙烯酸羟烷基酯凝胶亲和色谱法。II. 使用凝胶的汞衍生物分离含硫醇的蛋白质和肽。

Affinity chromatography on hydroxyalkyl methacrylate gels. II. Isolation of thiol-containing protein and peptide using mercurial derivatives of gels.

作者信息

Turková J, Vavreinová S, Kriváková M, Coupek J

出版信息

Biochim Biophys Acta. 1975 Apr 29;386(2):503-8. doi: 10.1016/0005-2795(75)90293-7.

DOI:10.1016/0005-2795(75)90293-7

Abstract

Absorption properties of hydroxyalkyl methacrylate gels containing various amounts of mercuri derivatives of p-acetaminophenoxyethyl methacrylates (Hg-APEMA) and methacrylanilide (Hg-MAA) were investigated by means of a reduced Ellman's agent (5,5'-dithiobis-2-nitrobenzoic acid) and papain. The optimum gel was used for the affinity chromatography of SH-protease from beans and for the isolation of a peptide with a free sulphhydryl group from the chymotryptic hydrolyzate of serum albumin.

摘要

通过还原型Ellman试剂（5,5'-二硫代双-2-硝基苯甲酸）和木瓜蛋白酶研究了含有不同量对乙酰氨基酚氧基乙基甲基丙烯酸酯（Hg-APEMA）和甲基丙烯酰苯胺（Hg-MAA）的汞衍生物的甲基丙烯酸羟烷基酯凝胶的吸收特性。最佳凝胶用于豆类中SH-蛋白酶的亲和色谱分析，以及从血清白蛋白的胰凝乳蛋白酶水解产物中分离具有游离巯基的肽。

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