Kanfer J N, Raghavan S S, Mumford R A
Biochim Biophys Acta. 1975 May 23;391(1):129-40. doi: 10.1016/0005-2744(75)90159-x.
Certain properties of the transglucosylitic activity and the hydrolytic activity of a purified calf spleen beta-glucosidase (beta-D-glucoside glucohydrolase EC 3.2.1.21) were investigated. There was a stimulation of both activities by sodium taurocholate and "Gaucher's factor". The K-m values for 4-methylumbelliferyl-beta-D-glucoside and glucosylceramide as donors in the transglucosylation reaction were 2 mM and 0.075 mM, respectively. The K-m for ceramide as acceptor was 0.149 mM with both of these compounds. The ability of several glucoside to act as donors was examined. The capacity to catalyze this "transglucosylation" reaction is greatly diminished in spleen tissue samples from Gaucher's patients. The enzyme possesses the capacity to hyrolyze 4-methylumbellifery-beta-D-glucoside, p-nitrophenyl-beta-D-glucoside, glucosylsphingosine, glucosylceramide and deoxycorticosterol-beta-D-glucoside. It is postulated that a single enzyme protein may be responsible for both the hydrolytic and the transglucosylitic activities.
对纯化的小牛脾脏β-葡萄糖苷酶（β-D-葡糖苷葡糖水解酶，EC 3.2.1.21）的转葡糖基活性和水解活性的某些特性进行了研究。牛磺胆酸钠和“高雪氏因子”对这两种活性均有刺激作用。在转葡糖基化反应中，4-甲基伞形酮基-β-D-葡糖苷和葡糖神经酰胺作为供体时的K-m值分别为2 mM和0.075 mM。对于这两种化合物，神经酰胺作为受体时的K-m为0.149 mM。研究了几种糖苷作为供体的能力。在高雪氏病患者的脾脏组织样本中，催化这种“转葡糖基化”反应的能力大大降低。该酶具有水解4-甲基伞形酮基-β-D-葡糖苷、对硝基苯基-β-D-葡糖苷、葡糖神经鞘氨醇、葡糖神经酰胺和脱氧皮质甾醇-β-D-葡糖苷的能力。据推测，单一的酶蛋白可能同时负责水解活性和转葡糖基活性。
