Rodrigues C S P, Souza S S, Rezende R P, Silva A, Andrioli J L, Costa H, Fontana R, Dias J C T
Laboratório de Monitoramento Ambiental, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Genet Mol Res. 2013 May 21;12(2):1752-60. doi: 10.4238/2013.May.21.6.
An estuary is a transition zone between freshwater and marine ecosystems, resulting in dilution of seawater. Estuaries are also considered environments of intense biological activity related to the processes of nutrient cycling. The aim of this study was to evaluate the microbial community composition along a salinity gradient in the estuary of the Cachoeira River, located in southern Bahia, Brazil. The analysis of bacterial and yeast communities was performed by determining the denaturing gradient gel electrophoresis band richness. Formation of zones with similar profiles of bands was observed, and the increasing richness at the intermediate zone demonstrated a clear spatial distinction of communities depending on salinity. In addition, the dissolved oxygen content, temperature, pH, salinity, and dissolved inorganic nutrient contents (NH3(+), NO2(-), NO3(-), PO4(-)) were determined. Nutrients were distributed in similar patterns, with decreasing concentrations as the salinity increases.
河口是淡水和海洋生态系统之间的过渡区域，会导致海水稀释。河口也被认为是与养分循环过程相关的生物活动强烈的环境。本研究的目的是评估位于巴西巴伊亚州南部的卡舒埃拉河河口沿盐度梯度的微生物群落组成。通过测定变性梯度凝胶电泳条带丰富度来分析细菌和酵母群落。观察到形成了具有相似条带图谱的区域，中间区域条带丰富度的增加表明群落根据盐度存在明显的空间差异。此外，还测定了溶解氧含量、温度、pH值、盐度和溶解无机养分含量（NH3(+)、NO2(-)、NO3(-)、PO4(-)）。养分以相似的模式分布，随着盐度增加浓度降低。
