Luczak Magdalena, Kaźmierczak Maciej, Hadschuh Luiza, Lewandowski Krzysztof, Komarnicki Mieczysław, Figlerowicz Marek
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Contemp Oncol (Pozn). 2012;16(2):95-103. doi: 10.5114/wo.2012.28787. Epub 2012 May 29.
The term proteomics was used for the first time in 1995 to describe large-scale protein analyses. At the same time proteomics was distinguished as a new domain of the life sciences. The major object of proteomic studies is the proteome, i.e. the set of all proteins accumulating in a given cell, tissue or organ. During the last years several new methods and techniques have been developed to increase the fidelity and efficacy of proteomic analyses. The most widely used are two-dimensional electrophoresis (2DE) and mass spectrometry (MS). In the past decade proteomic analyses have also been successfully applied in biomedical research. They allow one to determine how various diseases affect the pattern of protein accumulation. In this paper, we attempt to summarize the results of the proteomic analyses of acute myeloid leukemia (AML) cells. They have increased our knowledge on the mechanisms underlying AML development and contributed to progress in AML diagnostics and treatment.
“蛋白质组学”一词于1995年首次被用于描述大规模蛋白质分析。与此同时，蛋白质组学作为生命科学的一个新领域而被区分出来。蛋白质组学研究的主要对象是蛋白质组，即特定细胞、组织或器官中积累的所有蛋白质的集合。在过去几年中，已经开发了几种新的方法和技术来提高蛋白质组学分析的保真度和效率。使用最广泛的是二维电泳（2DE）和质谱（MS）。在过去十年中，蛋白质组学分析也已成功应用于生物医学研究。它们使人们能够确定各种疾病如何影响蛋白质积累模式。在本文中，我们试图总结急性髓系白血病（AML）细胞蛋白质组学分析的结果。这些结果增加了我们对AML发生机制的了解，并有助于AML诊断和治疗的进展。
