Bork Kaya, Hoffmann Matthias, Horstkorte Rüdiger
Institute for Physiological Chemistry, Martin-Luther University, Halle, Germany.
Neuroreport. 2013 Aug 7;24(11):616-9. doi: 10.1097/WNR.0b013e32836336c4.
The neural cell adhesion molecule (NCAM) is a member of the immunoglobulin superfamily and involved in the development and regeneration of the nervous system. NCAM is expressed in three major isoforms. Two of them have large intracellular domains of different lengths and are named according to their apparent molecular weight as NCAM140 or NCAM180. NCAM140 is known to promote neurite outgrowth, whereas NCAM180 stabilizes cell-cell contacts. Beside its role in neurite outgrowth, NCAM also shows ATPase activity on its extracellular domain. Here, we quantified neurite outgrowth of neuroblastoma cells, which were transfected with either NCAM140 or NCAM180 in the absence or presence of ATP. We could confirm that NCAM140 strongly promotes neurite outgrowth, whereas NCAM180 stimulates neurite outgrowth only to a minor extent. Furthermore, application of ATP reduced the NCAM-induced neurite outgrowth to background levels in a concentration-dependent and isoform-dependent manner.
神经细胞黏附分子（NCAM）是免疫球蛋白超家族的成员，参与神经系统的发育和再生。NCAM以三种主要异构体形式表达。其中两种具有不同长度的大细胞内结构域，并根据其表观分子量分别命名为NCAM140或NCAM180。已知NCAM140可促进神经突生长，而NCAM180可稳定细胞间接触。除了在神经突生长中的作用外，NCAM在其细胞外结构域还表现出ATP酶活性。在此，我们对在有无ATP的情况下转染了NCAM140或NCAM180的神经母细胞瘤细胞的神经突生长进行了定量。我们可以证实，NCAM140强烈促进神经突生长，而NCAM180仅在较小程度上刺激神经突生长。此外，ATP的应用以浓度依赖性和异构体依赖性方式将NCAM诱导的神经突生长降低至背景水平。
