Proliferative potential of murine melanoma cells cultured in or on collagen gel.

As the first step in developing an in vitro model of melanoma cells infiltrating the dermis, B16 murine melanoma cells were cultured on and in type I collagen gels. Under these conditions, the melanoma cell adopted an elongated or dendritic form. Cell proliferation was suppressed in the culture system using the collagen gel as compared with the conventional monolayer culture on plastic. Microcinematographically, this suppression was found to be due to an extension of the cell cycle time of each individual cell. On the other hand, there were no appreciable differences in proliferation pattern between the cells cultured on type I and IV collagen film and those cultured on plastic. These results suggest that there are interactions between type I collagen in the gel form and melanoma cells, especially with respect to cell growth.