Furuya Takeo, Hashimoto Masayuki, Koda Masao, Murata Atsushi, Okawa Akihiko, Dezawa Mari, Matsuse Dai, Tabata Yasuhiko, Takahashi Kazuhisa, Yamazaki Masashi
Chiba University Graduate School of Medicine, Chiba, Japan.
J Spinal Cord Med. 2013 Mar;36(2):134-9. doi: 10.1179/2045772312Y.0000000030.
Besides stimulating angiogenesis or cell survival, basic fibroblast growth factor (bFGF) has the potential for protecting neurons in the injured spinal cord.
To investigate the effects of a sustained-release system of bFGF from gelatin hydrogel (GH) in a rat spinal cord contusion model.
Adult female Sprague-Dawley rats were subjected to a spinal cord contusion injury at the T10 vertebral level using an IH impactor (200 kdyn). One week after contusion, GH containing bFGF (20 µg) was injected into the lesion epicenter (bFGF - GH group). The GH-only group was designated as the control. Locomotor recovery was assessed over 9 weeks by Basso, Beattie, Bresnahan rating scale, along with inclined plane and Rota-rod testing. Sensory abnormalities in the hind paws of all the rats were evaluated at 5, 7, and 9 weeks.
There were no significant differences in any of the motor assessments at any time point between the bFGF - GH group and the control GH group. The control GH group showed significantly more mechanical allodynia than did the group prior to injury. In contrast, the bFGF - GH group showed no statistically significant changes of mechanical withdrawal thresholds compared with pre-injury.
Our findings suggest that bFGF-incorporated GH could have therapeutic potential for alleviating mechanical allodynia following spinal cord injury.
碱性成纤维细胞生长因子（bFGF）除了具有刺激血管生成或促进细胞存活的作用外，还具有保护脊髓损伤后神经元的潜力。
在大鼠脊髓挫伤模型中研究明胶水凝胶（GH）缓释系统中bFGF的作用。
成年雌性Sprague-Dawley大鼠使用IH冲击器（200千达因）在T10椎体水平造成脊髓挫伤损伤。挫伤1周后，将含有bFGF（20μg）的GH注入损伤中心（bFGF - GH组）。仅注射GH的组作为对照组。通过Basso、Beattie、Bresnahan评分量表以及斜面和转棒试验在9周内评估运动功能恢复情况。在第5、7和9周评估所有大鼠后爪的感觉异常。
bFGF - GH组和对照GH组在任何时间点的任何运动评估中均无显著差异。对照GH组显示出比损伤前组明显更多的机械性异常性疼痛。相比之下，bFGF - GH组与损伤前相比，机械性退缩阈值无统计学显著变化。
我们的研究结果表明，含bFGF的GH可能具有治疗脊髓损伤后减轻机械性异常性疼痛的潜力。
