Department of Lipid Science and Traditional Foods, Central Food Technological Research Institute (CSIR), Mysore, 570 020, India.
Mol Cell Biochem. 2013 Oct;382(1-2):203-16. doi: 10.1007/s11010-013-1736-1. Epub 2013 Jun 30.
Long-chain n-3 fatty acids are essential for the development of cognitive functions and reducing the risk factor for cardiovascular diseases. The present study was undertaken to prepare fish oil (FO) microemulsion and explore the possibility of enhancing the enrichment of long-chain n-3 PUFA in the heart and brain lipids. The bioavailability of encapsulated FO was compared with that of native oil in rats by utilizing the intestinal sac method and by an in vivo study giving microemulsions of FO through intubation for a period of 30 days. Microemulsions were prepared using chitosan, gum acacia, whey protein, and lipoid. The bioavailability of eicosapentaenoic acid and docosahexaenoic acid (DHA) from FO encapsulated in chitosan, gum acacia, whey protein, and lipoid was increased by 7, 9, 23, and 68%, respectively, as compared to oil given without encapsulation in the everted intestinal sacs model. The DHA levels in serum lipids when FO was given as lipoid emulsion to rats were found to be 56 μg/ml, while rats given FO without encapsulation had a DHA level of 22 μg/ml. In the heart and brain lipids, the DHA levels were increased by 77 and 41%, respectively, in rats given FO encapsulated with lipoid compared to those given native oil. These studies indicated that DHA from FO was taken up in a more efficient manner when given in an encapsulated form with lipoid. Thus, phospholipid-based binding materials such as Lipoid provide a good delivery system for FO and significantly enhance DHA levels in the serum, liver, heart, and brain tissues.
长链 n-3 脂肪酸对于认知功能的发展和降低心血管疾病的风险因素至关重要。本研究旨在制备鱼油（FO）微乳液，并探索提高长链 n-3PUFA 在心脏和大脑脂质中富集的可能性。通过利用肠囊法和通过管饲给予 FO 微乳液 30 天的体内研究，比较了包封 FO 的生物利用度与天然油的生物利用度。微乳液是使用壳聚糖、瓜尔胶、乳清蛋白和脂质制备的。与未包封的油相比，包封在壳聚糖、瓜尔胶、乳清蛋白和脂质中的 EPA 和 DHA 的生物利用度分别提高了 7%、9%、23%和 68%。当 FO 以脂质体乳液给予大鼠时，血清脂质中的 DHA 水平为 56μg/ml，而给予未包封 FO 的大鼠的 DHA 水平为 22μg/ml。在心脏和大脑脂质中，与给予天然油的大鼠相比，给予脂质体包封的 FO 的大鼠的 DHA 水平分别增加了 77%和 41%。这些研究表明，当以脂质体的形式给予包封的 FO 时，DHA 被更有效地吸收。因此，基于磷脂的结合材料如 Lipoid 为 FO 提供了良好的递送系统，并显著提高了血清、肝脏、心脏和脑组织中的 DHA 水平。
