氰酸盐对凝血蛋白和血小板功能的影响。

The effect of cyanate on the clotting proteins and platelet function.

作者信息

Hilgartner M W, Miller D R

出版信息

Proc Soc Exp Biol Med. 1975 May;149(1):5-9. doi: 10.3181/00379727-149-38732.

Abstract

Cyanate reacts with unchanged amino groups of various proteins in a specific irreversible carbamylation reaction. The effect of this molecule on the clotting process and the effects of carbamylation on the clotting proteins and platelet functions were investigated in vitro. An immediate effect on the clotting proteins, not related to pH, was seen in the screening tests prothrombin time, partial thromboplastin time and thrombin time at the highest concentration (100 mM), to a lesser degree at the lower concentration (10 mM). These changes reflected decreases of 19 and 36% respectively in Factor V and X activity, an inhibition of 63-75% of Factors VII, IX, X and XI activity, and 80% inhibition of thrombin activity. The inhibitory changes of carbamylation increased with time. No changes were seen in the activity of Factors I and VIII. Platelet function studies revealed no inhibition of Factor III release; aggregation was abnormal only at high concentrations with epinephrine and collagen induction and partially reversible by resuspension in normal plasma.

摘要

氰酸盐在特定的不可逆氨甲酰化反应中与各种蛋白质未改变的氨基发生反应。在体外研究了该分子对凝血过程的影响以及氨甲酰化对凝血蛋白和血小板功能的影响。在凝血酶原时间、部分凝血活酶时间和凝血酶时间的筛选试验中，在最高浓度（100 mM）时可立即观察到对凝血蛋白的影响，且与pH无关，在较低浓度（10 mM）时影响较小。这些变化分别反映了因子V和X活性降低19%和36%，因子VII、IX、X和XI活性受到63 - 75%的抑制，凝血酶活性受到80%的抑制。氨甲酰化的抑制性变化随时间增加。因子I和VIII的活性未见变化。血小板功能研究显示因子III释放未受抑制；仅在高浓度下，肾上腺素和胶原蛋白诱导时聚集异常，且通过重悬于正常血浆中可部分逆转。