Department of Pulmonology and Allergology, Medical University of Lodz, Lodz, Poland.
Int J Tuberc Lung Dis. 2013 Aug;17(8):1082-7. doi: 10.5588/ijtld.12.0459.
The transmission of tuberculosis (TB) in the population and the development of disease are determined not only by the patient's immunological status, but also by the virulence of Mycobacterium tuberculosis strains.
To examine the virulence of M. tuberculosis clinical isolates with recognised transmission collected from 2006 to 2007 in a population in Lodz, Poland.
A total of 36 isolates were studied to determine their sensitivity to human neutrophil peptide 1 (HNP-1) and intracellular growth within THP-1 cells. Bacterial strains were cultured using HNP-1 at different concentrations. After incubation, the number of colony-forming units (cfu) was determined by bacteria plating. The intracellular survival was examined on days 3, 6 and 8 post-THP-1 infection by cfu enumeration.
Overall, 69% of the isolates showed greater resistance to the highest HNP-1 concentration (15 g/ml) than the virulent H37Rv strain, and the growth of 10 strains was totally inhibited. On day 8, 56% of the strains displayed higher cfu numbers than the virulent H37Rv strain.
The results suggest that isolates from our urban population represent highly virulent phenotypes. We could not find any significant difference in virulence between strains with unique genotypes and those in clusters.
人群中结核病（TB）的传播和疾病的发展不仅取决于患者的免疫状态，还取决于结核分枝杆菌菌株的毒力。
检测 2006 年至 2007 年期间在波兰罗兹市人群中分离的具有公认传播能力的结核分枝杆菌临床分离株的毒力。
共研究了 36 株分离株，以确定它们对人中性粒细胞肽 1（HNP-1）的敏感性和在 THP-1 细胞内的生长情况。使用不同浓度的 HNP-1 培养细菌株。孵育后，通过细菌平板计数确定集落形成单位（cfu）的数量。在 THP-1 感染后第 3、6 和 8 天，通过 cfu 计数检测细胞内存活情况。
总体而言，69%的分离株对最高 HNP-1 浓度（15μg/ml）的耐药性大于毒力较强的 H37Rv 株，且 10 株的生长完全受到抑制。在第 8 天，56%的菌株的 cfu 数量高于毒力较强的 H37Rv 株。
结果表明，我们从城市人群中分离的菌株代表了高度毒力的表型。我们没有发现基因型独特的菌株和聚类菌株之间的毒力存在显著差异。
Zotero 插件