大鼠肝细胞色素P450依赖性酶活性的体内诱导及其在培养中的维持。

The in vivo induction of rat hepatic cytochrome P450-dependent enzyme activities and their maintenance in culture.

作者信息

Hammond A H, Fry J R

机构信息

Department of Physiology and Pharmacology, Medical School, Queen's Medical Centre, Nottingham, U.K.

出版信息

Biochem Pharmacol. 1990 Aug 1;40(3):637-42. doi: 10.1016/0006-2952(90)90567-5.

DOI:10.1016/0006-2952(90)90567-5

PMID:2383291

Abstract

Cytochrome P450-dependent enzyme activities were measured in hepatocytes from adult male rats, induced in vivo with phenobarbitone, beta-naphthoflavone, dexamethasone or isoniazid: the stability of the induced activities in culture was also determined. Each inducer produced a characteristically different pattern of enzyme activities with dexamethasone, isoniazid and beta-naphthoflavone selectively inducing erythromycin N-demethylase, p-nitrophenol hydroxylase and ethoxyresorufin O-dealkylase respectively. In general, the induced activities were maintained for 24 hr in culture. This indicates the feasibility of an in vivo induction-hepatocyte culture system for the study of metabolism-mediated toxicity.

摘要

在成年雄性大鼠的肝细胞中测定细胞色素P450依赖性酶活性，这些大鼠在体内分别用苯巴比妥、β-萘黄酮、地塞米松或异烟肼诱导：还测定了诱导活性在培养中的稳定性。每种诱导剂产生的酶活性模式都有显著差异，地塞米松、异烟肼和β-萘黄酮分别选择性诱导红霉素N-脱甲基酶、对硝基苯酚羟化酶和乙氧基试卤灵O-脱烷基酶。一般来说，诱导活性在培养中维持24小时。这表明体内诱导-肝细胞培养系统用于研究代谢介导毒性的可行性。