放线菌素D对鸟氨酸脱羧酶（ODC）的超诱导作用是由于刺激了ODC信使核糖核酸的翻译。

Superinduction of ornithine decarboxylase (ODC) by actinomycin D is due to stimulation of ODC mRNA translation.

作者信息

Wallon U M, Persson L, Heby O

机构信息

Department of Zoophysiology, University of Lund, Sweden.

出版信息

FEBS Lett. 1990 Jul 30;268(1):161-4. doi: 10.1016/0014-5793(90)80998-x.

DOI:10.1016/0014-5793(90)80998-x

PMID:2384152

Abstract

Inhibition of transcription by treatment with actinomycin D caused superinduction of the ornithine decarboxylase (ODC) activity in Ehrlich ascites tumor cells. Experiments with cycloheximide ruled out the possibility that this superinduction was due to stabilization of ODC. Instead the ODC activity exhibited a more rapid turnover in the presence of actinomycin D (t1/2 = 56 min). The superinduction was found to coincide with an increased rate of ODC synthesis, as determined by measuring the incorporation of [35S )methionine into immunoreactive ODC protein. The steady-state level of ODC mRNA was unchanged, indicating an effect on the translational efficiency.

摘要

用放线菌素D处理抑制转录会导致艾氏腹水瘤细胞中鸟氨酸脱羧酶（ODC）活性的超诱导。用环己酰亚胺进行的实验排除了这种超诱导是由于ODC稳定化的可能性。相反，在放线菌素D存在的情况下，ODC活性表现出更快的周转（t1/2 = 56分钟）。通过测量[35S]甲硫氨酸掺入免疫反应性ODC蛋白中确定，发现超诱导与ODC合成速率增加相吻合。ODC mRNA的稳态水平未改变，表明对翻译效率有影响。