Department of Pharmacokinetics and Pharmacodynamics, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
J Pharmacol Sci. 2013;122(4):270-7. doi: 10.1254/jphs.13075fp. Epub 2013 Jul 11.
The aim of this study was to characterize pharmacological effects of gosha-jinki-gan (GJG) and green tea extract (GTE), on urodynamic parameters, bladder receptors, and urinary cytokines in rats with cyclophosphamide (CYP)-induced cystitis. Urodynamic parameters in CYP-treated rats were measured using the cystometric method. Muscarinic and purinergic receptors in rat tissues were measured by radioreceptor assays. Urinary cytokine levels were measured with ELISA kits. GJG and GTE were orally administered to rats once a day for 7 days. The GJG treatment significantly ameliorated changes in urodynamic parameters in CYP-treated rats. Similar treatment with GTE slightly attenuated changes in urodynamic parameters. The maximal number of binding sites for [³H]NMS and [³H]αβ-MeATP in the bladder was significantly lower in CYP-treated rats than in sham rats. Such a reduction in receptor density was significantly attenuated by the GJG treatment. GTE treatment also significantly attenuated the down-regulation of muscarinic receptors, but not P2X receptors in bladders of rats with CYP-induced cystitis. The elevation in urinary cytokine levels in CYP-treated rats was effectively attenuated by GJG treatment. The elevation in cytokine levels in CYP-treated rats was alleviated by GTE treatment. In conclusion, GJG may be a pharmacologically useful plant extract for cystitis.
本研究旨在探讨苦参金感汤（GJG）和绿茶提取物（GTE）对环磷酰胺（CYP）诱导膀胱炎大鼠尿动力学参数、膀胱受体和尿细胞因子的药理作用。采用膀胱测压法测定 CYP 处理大鼠的尿动力学参数。通过放射受体测定法测定大鼠组织中的毒蕈碱和嘌呤能受体。采用 ELISA 试剂盒测定尿细胞因子水平。GJG 和 GTE 每天口服一次，连续 7 天。GJG 治疗可显著改善 CYP 处理大鼠的尿动力学参数变化。用 GTE 进行类似的治疗可轻微减轻尿动力学参数的变化。[³H]NMS 和 [³H]αβ-MeATP 在膀胱中的最大结合位点数量在 CYP 处理的大鼠中明显低于假手术大鼠。GJG 治疗可显著减轻这种受体密度的降低。GTE 治疗还可显著减轻 CYP 诱导膀胱炎大鼠膀胱中毒蕈碱受体的下调，但对 P2X 受体无影响。GJG 治疗可有效减轻 CYP 处理大鼠尿细胞因子水平的升高。GTE 治疗可减轻 CYP 处理大鼠细胞因子水平的升高。综上所述，GJG 可能是一种治疗膀胱炎的有价值的植物提取物。
