Department of Radiology, Northwestern University, United States; Robert H. Lurie Comprehensive Cancer Center, Northwestern University, United States.
J Immunol Methods. 2013 Dec 31;400-401:23-9. doi: 10.1016/j.jim.2013.06.013. Epub 2013 Jul 12.
Mouse models are fundamental to the study and design of new techniques for the cancer diagnosis and treatment. The lymphatic system plays an active role in oncogenesis and metastatic disease progression. However, the in vivo identification of LNs in mice is challenging with conventional imaging modalities since the LN diameter in normal mice is 1-2 mm. Standard dissection techniques are challenging and can only provide endpoint data. Here, we describe high resolution MRI (HR-MRI) approaches for the non-invasive detection of mouse LNs in vivo. We compare in vivo non-invasive HR-MRI methods (without exogenous contrast injections) to the ex vivo dye injection methods for the identification of commonly studied LNs in both normal mice and a mouse model of pancreatic ductal adenocarcinoma (PDAC). We demonstrated the potential to use HR-MRI techniques as a non-invasive imaging assay for visualizing mouse LNs in vivo.
小鼠模型是癌症诊断和治疗新技术研究和设计的基础。淋巴系统在肿瘤发生和转移疾病进展中起着积极的作用。然而，由于正常小鼠的淋巴结直径为 1-2 毫米，传统的成像方式在体内识别 LNs 具有挑战性。标准的解剖技术具有挑战性，只能提供终点数据。在这里，我们描述了用于体内非侵入性检测小鼠 LNs 的高分辨率 MRI（HR-MRI）方法。我们将体内非侵入性 HR-MRI 方法（无需外源性对比注射）与外源性染料注射方法进行比较，以确定正常小鼠和胰腺导管腺癌（PDAC）小鼠模型中常见研究的 LNs。我们证明了使用 HR-MRI 技术作为一种非侵入性成像测定方法，用于体内可视化小鼠 LNs 的潜力。
