Soares Filippe Elias de Freitas, Braga Fabio Ribeiro, Araújo Jackson Victor de, Lima Walter dos Santos, Mozzer Lanuze Rose, Queiroz José Humberto de
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Rev Bras Parasitol Vet. 2013 Apr-Jun;22(2):285-8. doi: 10.1590/S1984-29612013000200048.
The objectives of this study were to optimize protease production from the nematophagous fungus Monacrosporium thaumasium (NF34a) and evaluate its larvicidal activity and biological stability. An isolate of the nematophagous fungus Monacrosporium thaumasium (NF34a) was used to produce the enzyme. The Plackett-Burman design was used in order to scan which components of the culture medium could have a significant influence on protease production by the fungus NF34a. An in vitro assay was also performed to evaluate the larvicidal activity of NF34a. It was observed that only one component of the culture medium (yeast extract), at the levels studied, had any significant effect (p < 0.05) on protease production. There was a reduction (p < 0.01) in the mean number of larvae recovered from the treated groups, compared with the control groups. The results confirm previous reports on the efficiency of nematophagous fungi for controlling nematode larvae that are potentially zoonotic. Thus, given the importance of biological control, we suggest that further studies should be conducted on the protease produced by the fungus Monacrosporium thaumasium.
本研究的目的是优化食线虫真菌奇妙单顶孢（NF34a）的蛋白酶产量，并评估其杀幼虫活性和生物稳定性。使用食线虫真菌奇妙单顶孢（NF34a）的一个分离株来生产该酶。采用Plackett-Burman设计以筛选培养基的哪些成分可能对真菌NF34a产生蛋白酶有显著影响。还进行了体外试验以评估NF34a的杀幼虫活性。观察到在所研究的水平下，培养基中只有一种成分（酵母提取物）对蛋白酶产量有任何显著影响（p < 0.05）。与对照组相比，处理组回收的幼虫平均数量有所减少（p < 0.01）。结果证实了先前关于食线虫真菌控制具有潜在人畜共患病风险的线虫幼虫效率的报道。因此，鉴于生物防治的重要性，我们建议应对奇妙单顶孢真菌产生的蛋白酶进行进一步研究。
