Hoang Dung Minh, Voura Evelyn B, Zhang Chao, Fakri-Bouchet Latifa, Wadghiri Youssef Zaim
The Bernard & Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University Langone Medical Center (NYULMC), New York, New York, USA; Creatis-LRMN, UMR CNRS 5220, INSERM U 630, Université Lyon 1, Villeurbanne, France.
Magn Reson Med. 2014 May;71(5):1932-43. doi: 10.1002/mrm.24841. Epub 2013 Jul 15.
To investigate the relative gain in sensitivity of five histology coils designed in-house to accommodate tissue sections of various sizes and compare with commercial mouse head coils.
The coil set was tailored to house tissue sections ranging from 5 to1000 µm encased in either glass slides or coverslips.
Our simulations and experimental measurements demonstrated that although the sensitivity of this flat structure consistently underperforms relative to a birdcage head coil based on the gain expected from their respective filling factor ratios, our results demonstrate that it can still provide a remarkable gain in sensitivity. Our study also describes preparation protocols for freshly excised sections, as well as premounted tissue slides of both mouse and human specimens. Examples of the exceptional level of tissue detail and the near-perfect magnetic resonance imaging to light microscopic image coregistration are provided.
The increase in filling factor achieved by the histology radiofrequency (RF) probe overcomes the losses associated with electric leaks inherent to this structure, leading to a 6.7-fold improvement in performance for the smallest coil implemented. Alternatively, the largest histology coil design exhibited equal sensitivity to the mouse head coil while nearly doubling the RF planar area coverage.
研究自行设计的五个组织学线圈在容纳不同尺寸组织切片时的相对灵敏度增益，并与商用小鼠头部线圈进行比较。
该线圈组经过定制，可容纳封装在载玻片或盖玻片中、厚度从5到1000微米的组织切片。
我们的模拟和实验测量表明，尽管基于各自填充因子比预期的增益，这种扁平结构的灵敏度始终低于鸟笼式头部线圈，但我们的结果表明它仍能提供显著的灵敏度增益。我们的研究还描述了新鲜切除切片以及小鼠和人类标本预安装组织切片的制备方案。提供了组织细节的卓越水平以及近乎完美的磁共振成像与光学显微镜图像配准的示例。
组织学射频（RF）探头实现的填充因子增加克服了与该结构固有的电泄漏相关的损耗，使最小的线圈性能提高了6.7倍。或者，最大的组织学线圈设计对小鼠头部线圈表现出相同的灵敏度，同时射频平面面积覆盖率几乎增加了一倍。
