Kousar Samina, Sheikh Munir Ahmad, Asghar Muhammad
Department of Biochemistry, University College of Medicine, University of Lahore, Pakistan.
J Pak Med Assoc. 2012 Oct;62(10):1033-8.
To observe the antiglycation effect of water-soluble vitamin, thiamin-hydrochloride (B1) and its fat-soluble derivative benfotiamine and their comparison with two different glycation assays in diabetics.
Plasma of both the normal's and diabetic persons was taken for this experimental study which was conducted in the Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad in 2008. Varying concentrations of both the glycation inhibitors and glucose were incubated for 5 weeks at 37 degrees C. Thiobarbituric acid and periodate borohydride assays were used to measure the antiglycation activity.
The increase in glycation was observed from 1st to 3rd week of incubation, while it decreased after the 5th week due to the formation of advanced glycation end products.
It was observed that 10mM concentration of benfotiamine and 5mM and 1mM concentrations of thiamine-hydrochloride produced fairly good response to decreased glycation. Comparison between two assays proved periodate borohydride to be more reliable and sensitive than thiobarbituric acid.
观察水溶性维生素盐酸硫胺素（B1）及其脂溶性衍生物苯磷硫胺的抗糖基化作用，并将它们与糖尿病患者中两种不同的糖基化检测方法进行比较。
本实验研究采用正常人和糖尿病患者的血浆，于2008年在费萨拉巴德农业大学化学与生物化学系进行。将不同浓度的糖基化抑制剂和葡萄糖在37℃下孵育5周。采用硫代巴比妥酸法和高碘酸盐硼氢化物法测定抗糖基化活性。
在孵育的第1至3周观察到糖基化增加，而在第5周后由于晚期糖基化终产物的形成糖基化减少。
观察到10mM浓度的苯磷硫胺以及5mM和1mM浓度的盐酸硫胺素对降低糖基化有相当好的反应。两种检测方法的比较证明，高碘酸盐硼氢化物法比硫代巴比妥酸法更可靠、更灵敏。
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