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从韩国水稻品种中分离和鉴定具有促生长功能的内生固氮细菌。

Isolation and characterization of plant growth promoting endophytic diazotrophic bacteria from Korean rice cultivars.

机构信息

Department of Molecular Biotechnology, Konkuk University, Seoul 143-701, Republic of Korea.

出版信息

Microbiol Res. 2014 Jan 20;169(1):83-98. doi: 10.1016/j.micres.2013.06.003. Epub 2013 Jul 17.

Abstract

We have isolated 576 endophytic bacteria from the leaves, stems, and roots of 10 rice cultivars and identified 12 of them as diazotrophic bacteria using a specific primer set of nif gene. Through 16S rDNA sequence analysis, nifH genes were confirmed in the two species of Penibacillus, three species of Microbacterium, three Bacillus species, and four species of Klebsiella. Rice seeds treated with these plant growth-promoting bacteria (PGPB) showed improved plant growth, increased height and dry weight and antagonistic effects against fungal pathogens. In addition, auxin and siderophore producing ability, and phosphate solubilizing activity were studied for the possible mechanisms of plant growth promotion. Among 12 isolates tested, 10 strains have shown higher auxin producing activity, 6 isolates were confirmed as strains with high siderophore producing activity while 4 isolates turned out to have high phosphate-solubilizing activity. These results strongly suggest that the endophytic diazotrophic bacteria characterized in this study could be successfully used to promote plant growth and inducing fungal resistance in plants.

摘要

我们从 10 个水稻品种的叶片、茎和根部分离出 576 株内生细菌,并使用 nif 基因的特定引物对鉴定出其中的 12 株为固氮细菌。通过 16S rDNA 序列分析,在 2 种 Penibacillus 、3 种 Microbacterium 、3 种 Bacillus 和 4 种 Klebsiella 物种中证实了 nifH 基因的存在。用这些植物促生菌(PGPB)处理的水稻种子表现出更好的植物生长、增加了高度和干重,并对真菌病原体表现出拮抗作用。此外,还研究了生长素和铁载体产生能力以及磷酸盐溶解活性,以探究植物生长促进的可能机制。在测试的 12 个分离株中,有 10 株表现出更高的生长素产生活性,6 株被确认为具有高铁载体产生活性的菌株,而 4 株则表现出高磷酸盐溶解活性。这些结果强烈表明,本研究中所描述的内生固氮细菌可以成功地用于促进植物生长和诱导植物对真菌的抗性。

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