Guérin C J, Anderson D H, Fisher S K
Neuroscience Research Institute, University of California, Santa Barbara 93106.
Invest Ophthalmol Vis Sci. 1990 Aug;31(8):1474-82.
The immunolabeling patterns for vimentin and glial fibrillary acidic protein (GFAP) were studied in five rhesus monkeys that had undergone retinal detachment or detachment and reattachment. Anti-vimentin and anti-GFAP labeling intensity increased in Müller cells after 2 days of detachment. Weak anti-vimentin labeling of the basal RPE cytoplasm, which was absent in control tissue, was detected 2 days after detachment. After detachment for 7 days and reattachment for 7 or 14 days, the pattern and extent of intermediate filament (IF) labeling changed. In Müller cells, the labeling, which in controls was restricted to processes near the vitreal border of the retina, was present in Müller processes spanning the entire retina. In retinal pigment epithelium (RPE) cells, prominent anti-vimentin labeling was identified in the basal and basolateral cytoplasm. The extent of RPE and Müller cell IF labeling in two animals whose retinas had been detached and then reattached for 150 days was different from that found at either the 7- or 14-day reattachment time points. This suggests that the abnormal IF distribution triggered by detachment may be attenuated after a lengthy period of reattachment.
在五只经历过视网膜脱离或脱离并复位的恒河猴中，研究了波形蛋白和胶质纤维酸性蛋白（GFAP）的免疫标记模式。脱离2天后，Müller细胞中波形蛋白和GFAP的抗标记强度增加。脱离2天后，检测到视网膜色素上皮（RPE）基底细胞质中有微弱的波形蛋白抗标记，而对照组织中不存在这种情况。脱离7天并复位7或14天后，中间丝（IF）标记的模式和范围发生了变化。在Müller细胞中，对照时局限于视网膜玻璃体边界附近突起的标记，在横跨整个视网膜的Müller突起中也有出现。在视网膜色素上皮（RPE）细胞中，在基底和基底外侧细胞质中发现了明显的波形蛋白抗标记。两只视网膜脱离然后复位150天的动物中，RPE和Müller细胞IF标记的范围与复位7天或14天时发现的情况不同。这表明，由脱离引发的异常IF分布可能在长时间复位后减弱。
