Department of Food, Agricultural and Biological Engineering, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, OH 44691, USA.
Bioresour Technol. 2013 Sep;144:281-7. doi: 10.1016/j.biortech.2013.06.106. Epub 2013 Jul 2.
The inhibitive effect of total ammonia nitrogen (TAN) (including NH3 and NH4(+)) on solid-state anaerobic digestion of corn stover was investigated in batch reactors at 37°C. The highest methane yield of 107.0 L/kg VS(feed) was obtained at a TAN concentration of 2.5 g/kg (based on total weight). TAN concentrations greater than 2.5 g/kg resulted in decreased methane yields, with a 50% reduction observed at a concentration of 6.0 g/kg. Reduced reaction rates and microbial activities for hydrolysis of cellulose and methanogenesis from acetate were observed at TAN concentrations higher than 4.3 g/kg. Strong ammonia stress was indicated at butyrate concentrations higher than 300 mg/kg. Result showed that the effluent of liquid anaerobic digestion can provide enough nitrogen for solid-state anaerobic digestion of corn stover.
在 37°C 的分批式反应器中,考察了总氨氮(TAN)(包括 NH3 和 NH4(+))对玉米秸秆固态厌氧消化的抑制作用。在 TAN 浓度为 2.5 g/kg(基于总重量)时,获得了最高的甲烷产率 107.0 L/kg VS(进料)。TAN 浓度大于 2.5 g/kg 会导致甲烷产率降低,在浓度为 6.0 g/kg 时,甲烷产率降低了 50%。在 TAN 浓度高于 4.3 g/kg 时,观察到纤维素水解和乙酸盐产甲烷的反应速率和微生物活性降低。丁酸浓度高于 300 mg/kg 时,表明存在强烈的氨胁迫。结果表明,液体厌氧消化的流出物可为玉米秸秆的固态厌氧消化提供足够的氮。