Holmgren S, Nilsson S
Eur J Pharmacol. 1975 Jun-Jul;32(02):163-9. doi: 10.1016/0014-2999(75)90279-4.
The effects of drugs on isolated spleen strips from the cod, Gadus morhua, have been investigated. Affinities and intrinsic activities for various agonists were determined from cumulative dose-response curves. Contractions were produced by acetylcholine, methacholine or carbachol. The response to acetylcholine was considerably potentiated by the acetylcholinesterase inhibitor BW 284 C51. Methacholine-induced responses were competitively blocked by atropine (pA2 = 8.2), indicating the presence of muscarinic receptors. Nicotine did not contract the spleen strips. Adrenaline, noradrenaline or phenylephrine contracted the preparations and so did isoprenaline in high concentrations. Competitive blockade of the noradrenaline-induced contraction was obtained with yohimbine (pA2 = 6.1), phentolamine (pA2 = 6.0) and propranolol (pA2 = 3.4), and non-competitive blockade with phenoxybenzamine (pM50 = 7.0), indicating that alpha-adrenoceptors mediate the contractions produced by the adrenergic agonists. Isoprenaline caused a slight relaxation of preparations precontracted with methacholine. This effect was abolished by propranolol suggesting the presence of beta-adrenoceptors. Judged from the pA-values for the competitive antagonist, arterial and capsular/trabecular smooth muscles have slightly different alpha-adrenoceptor properties.
已对药物对鳕鱼（Gadus morhua）离体脾条的作用进行了研究。通过累积剂量-反应曲线确定了各种激动剂的亲和力和内在活性。乙酰胆碱、醋甲胆碱或卡巴胆碱可引起收缩。乙酰胆碱酯酶抑制剂BW 284 C51可显著增强对乙酰胆碱的反应。阿托品竞争性阻断醋甲胆碱诱导的反应（pA2 = 8.2），表明存在毒蕈碱受体。尼古丁不会使脾条收缩。肾上腺素、去甲肾上腺素或去氧肾上腺素可使制剂收缩，高浓度的异丙肾上腺素也可使其收缩。育亨宾（pA2 = 6.1）、酚妥拉明（pA2 = 6.0）和普萘洛尔（pA2 = 3.4）可竞争性阻断去甲肾上腺素诱导的收缩，苯氧苄胺（pM50 = 7.0）可产生非竞争性阻断，表明α-肾上腺素受体介导了肾上腺素能激动剂产生的收缩。异丙肾上腺素可使预先用醋甲胆碱收缩的制剂略有松弛。普萘洛尔可消除这种效应，提示存在β-肾上腺素受体。从竞争性拮抗剂的pA值判断，动脉平滑肌和被膜/小梁平滑肌的α-肾上腺素受体特性略有不同。
