经肝内接种实验大鼠，研究基因 4 型戊型肝炎病毒 cDNA 克隆的感染性。

Infectivity of a genotype 4 hepatitis E virus cDNA clone by intrahepatic inoculation of laboratory rats.

机构信息

Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agricultural Sciences, Shanghai, China.

出版信息

Vet Microbiol. 2013 Oct 25;166(3-4):405-11. doi: 10.1016/j.vetmic.2013.06.021. Epub 2013 Jul 9.

DOI:10.1016/j.vetmic.2013.06.021

Abstract

A cDNA clone of a genotype 4 swine hepatitis E virus (HEV) strain (SAAS-FX17), identified in Shanghai, has been constructed. Capped RNA transcripts were prepared in vitro and shown to be replication-competent in Huh7 cells. Sprague-Dawley (SD) rats administered the RNA transcripts by intrahepatic inoculation developed active infections as evidenced by fecal virus shedding and sero-conversion to anti-HEV. The former was first detected between 23 and 30 days post-inoculation (dpi) and persisted until 45 dpi. Sera of rats inoculated with RNA transcripts became anti-HEV positive between 30 and 40 dpi, and reverted to anti-HEV negative at 52 dpi. Our data indicate for the first time that intrahepatic inoculation of rats with RNA transcripts of an HEV cDNA clone may serve as an alternative animal model for HEV research.

摘要

构建了一株上海地区分离的基因型 4 猪乙型肝炎病毒（HEV）SAAS-FX17 cDNA 克隆。体外制备了加帽的 RNA 转录本，并在 Huh7 细胞中显示出具有复制能力。经肝内接种 RNA 转录本的 Sprague-Dawley（SD）大鼠出现了活跃的感染，粪便病毒排出和抗-HEV 血清转换可证明这一点。前者最早在接种后 23 至 30 天（dpi）检测到，并持续到 45 dpi。接种 RNA 转录本的大鼠血清在 30 至 40 dpi 之间变为抗-HEV 阳性，并在 52 dpi 时恢复为抗-HEV 阴性。我们的数据首次表明，用 HEV cDNA 克隆的 RNA 转录本肝内接种大鼠可能成为替代的 HEV 研究动物模型。