三丁基氯化锡诱导的HEL-30细胞蛋白质合成抑制在ATP恢复后的可逆性。

Reversibility of tributyltin-chloride-induced protein synthesis inhibition after ATP recovery in HEL-30 cells.

作者信息

Marinovich M, Viviani B, Galli C L

机构信息

Institute of Pharmacological Sciences, University of Milan, Italy.

出版信息

Toxicol Lett. 1990 Aug;52(3):311-7. doi: 10.1016/0378-4274(90)90041-j.

DOI:10.1016/0378-4274(90)90041-j

PMID:2389259

Abstract

The effect of tributyltin chloride (TBT) on ATP levels and protein synthesis was investigated in a murine epidermal cell line (HEL-30). Five minutes after exposure to 10(-5) M TBT, cellular levels of ATP decreased by 57% and protein synthesis was significantly inhibited. The effect of ATP was stable up to 2 h incubation, whereas inhibition of protein synthesis increased with time of treatment. Partial (1 h) or complete (2 h) recovery of the two cell functions was observed when dithiothreitol (DTT) was added to the medium, 5 min after the damage and in the absence of TBT. The sequence of the observed effects suggests that TBT inhibition of protein synthesis is due to impairment of ATP production.

摘要

研究了三丁基氯化锡（TBT）对小鼠表皮细胞系（HEL-30）中ATP水平和蛋白质合成的影响。暴露于10^(-5) M TBT 5分钟后，细胞内ATP水平下降了57%，蛋白质合成受到显著抑制。ATP的这种影响在长达2小时的孵育过程中保持稳定，而蛋白质合成的抑制随着处理时间的延长而增加。在损伤后5分钟且不存在TBT的情况下，向培养基中添加二硫苏糖醇（DTT）时，观察到这两种细胞功能部分（1小时）或完全（2小时）恢复。观察到的效应顺序表明，TBT对蛋白质合成的抑制是由于ATP生成受损所致。