在不同时间使用不同剂量的三碘甲状腺原氨酸（T3）对甲状腺激素受体TRα和TRβ进行调节。
Modulation of thyroid hormone receptors, TRα and TRβ, by using different doses of triiodothyronine (T3) at different times.
作者信息
Oliveira Miriane de, Luvizotto Renata de Azevedo Melo, Olimpio Regiane Marques Castro, Sibio Maria Teresa de, Silva Carolina Biz Rodrigues, Conde Sandro José, Padovani Carlos Roberto, Nogueira Célia Regina
机构信息
Department of Internal Clinic, Botucatu Medicine School, São Paulo State University, Botucatu, SP, Brazil.
出版信息
Arq Bras Endocrinol Metabol. 2013 Jul;57(5):368-74. doi: 10.1590/s0004-27302013000500006.
OBJECTIVE
To examine the effect of different doses of triiodothyronine (T3) on mRNA levels of thyroid hormone receptors, TRα and TRβ, at different times.
MATERIALS AND METHODS
3T3-L1 adipocytes were incubated with T3 (physiological dose: F; supraphysiological doses: SI or SII), or without T3 (control, C) for 0.5, 1, 6, or 24h. TRα and TRβ mRNA was detected using real-time polymerase chain reaction.
RESULTS
F increased TRβ mRNA levels at 0.5h. After 1h, TRα levels increased with F and SI and TRβ levels decreased with SII compared with C, F, and SI. After 6h, both genes were suppressed at all concentrations. In 24h, TRα and TRβ levels were similar to those of C group.
CONCLUSIONS
T3 action with F began at 1h for TRα and at 0.5h for TRβ. These results suggest the importance of knowing the times and doses that activate T3 receptors in adipocytes.
目的
研究不同剂量的三碘甲状腺原氨酸（T3）在不同时间对甲状腺激素受体TRα和TRβ的mRNA水平的影响。
材料与方法
将3T3-L1脂肪细胞与T3（生理剂量：F；超生理剂量：SI或SII）孵育，或不与T3孵育（对照，C）0.5、1、6或24小时。使用实时聚合酶链反应检测TRα和TRβ mRNA。
结果
F在0.5小时时增加TRβ mRNA水平。1小时后，与C、F和SI相比，F和SI使TRα水平升高，SII使TRβ水平降低。6小时后，所有浓度下两个基因均被抑制。24小时时，TRα和TRβ水平与C组相似。
结论
F对TRα的T3作用在1小时开始，对TRβ的作用在0.5小时开始。这些结果表明了解激活脂肪细胞中T3受体的时间和剂量的重要性。
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