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重复序列注释与从头重复序列鉴定概述。

Overview of repeat annotation and de novo repeat identification.

作者信息

Jiang Ning

机构信息

Department of Horticulture, Michigan State University, East Lansing, MI, USA.

出版信息

Methods Mol Biol. 2013;1057:275-87. doi: 10.1007/978-1-62703-568-2_20.

Abstract

The availability of a large amount of genomic sequences has provided unique opportunities for understanding the composition and dynamics of transposable elements (TEs) in plants. As the cost of sequencing declines, the genomic sequences of most crop plants will be available within the next few years. Thus, the annotation of genomic sequences, rather than sequence availability, will become the "bottleneck" for genome study. Since TEs are the largest component of most plant genomes, the automation of TE identification and classification is essential for future genome annotation as well as characterization of TEs. In this chapter, the functions and mechanisms of different repeat finding tools are reviewed, with a focus on de novo repeat identification programs. In addition, this chapter covers the further processing of results from de novo identification programs and the construction of repeat libraries for downstream genome analyses.

摘要

大量基因组序列的可得性为理解植物中转座元件(TEs)的组成和动态提供了独特的机会。随着测序成本的下降,大多数农作物的基因组序列将在未来几年内可得。因此,基因组序列的注释,而非序列的可得性,将成为基因组研究的“瓶颈”。由于TEs是大多数植物基因组的最大组成部分,TE识别和分类的自动化对于未来的基因组注释以及TEs的特征描述至关重要。在本章中,将对不同重复序列查找工具的功能和机制进行综述,重点是从头重复序列识别程序。此外,本章还涵盖了从头识别程序结果的进一步处理以及用于下游基因组分析的重复序列文库的构建。

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