Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Collegium Medicum, Jagiellonian University, 9 Medyczna Str, 30-688 Kraków, Poland.
J Pharm Biomed Anal. 2013 Nov;85:132-7. doi: 10.1016/j.jpba.2013.07.017. Epub 2013 Jul 25.
The chromatographic behavior of a series of coxibs and oxicams, drugs from a group of non-steroidal anti-inflammatory drugs, was studied by reversed-phase thin-layer chromatography with binary mobile phases containing water and the organic modifiers: methanol, acetone, 1,4-dioxane, acetonitrile and 2-propanol. Linear relationships were obtained between the retention RM values of the compounds and the concentration of organic modifier in the mobile phase. Values of RM0, represent the theoretical RM values at 0% organic solvent in the mobile phase were calculated by extrapolation. These experimental lipophilicity values were correlated with lipophilicity (logP) from databases. The obtained results show that reversed-phase chromatography (experimental parameters) may be a good instrument for analytics in describing the lipophilic nature of investigated compounds as well as the activity.
采用反相薄层色谱法，以水和甲醇、丙酮、1,4-二氧六环、乙腈和异丙醇等有机溶剂组成的二元流动相作为展开剂，对一系列昔布类和昔布类药物（非甾体类抗炎药的一个类别）进行了色谱行为研究。化合物的保留 RM 值与流动相中有机溶剂浓度之间存在线性关系。通过外推法计算出 RM0 值，代表在流动相中有机溶剂浓度为 0%时的理论 RM 值。这些实验的亲脂性值与数据库中的亲脂性（logP）相关联。结果表明，反相色谱（实验参数）可以作为一种良好的分析仪器，用于描述所研究化合物的亲脂性本质以及活性。
