转化生长因子α-抗 Tac（Fv）-PE40：一种对具有表皮生长因子或白细胞介素 2 受体的细胞具有细胞毒性的双功能毒素。

TGF alpha-anti-Tac(Fv)-PE40: a bifunctional toxin cytotoxic for cells with EGF or IL2 receptors.

作者信息

Batra J K, Chaudhary V K, FitzGerald D, Pastan I

机构信息

Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.

出版信息

Biochem Biophys Res Commun. 1990 Aug 31;171(1):1-6. doi: 10.1016/0006-291x(90)91347-u.

DOI:10.1016/0006-291x(90)91347-u

PMID:2393383

Abstract

Conventional immunotoxins and chimeric toxins made in bacteria are directed to only one receptor or antigen on target cells. In this report we describe the construction of a chimeric molecule TGF alpha-anti Tac(Fv)-PE40 which is composed of human transforming growth factor type alpha attached to anti-Tac(Fv) which is in turn attached to PE40, a form of pseudomonas exotoxin, devoid of its cell recognition domain. TGF alpha-anti-Tac(Fv)-PE40 is a bifunctional toxin that is produced in E. coli and is active on cells bearing either IL2 or EGF receptors.

摘要

在细菌中制备的传统免疫毒素和嵌合毒素仅作用于靶细胞上的一种受体或抗原。在本报告中，我们描述了一种嵌合分子TGFα-抗Tac（Fv）-PE40的构建，它由与抗Tac（Fv）相连的人α型转化生长因子组成，而抗Tac（Fv）又与缺乏细胞识别结构域的假单胞菌外毒素形式PE40相连。TGFα-抗Tac（Fv）-PE40是一种双功能毒素，在大肠杆菌中产生，对携带IL2或EGF受体的细胞具有活性。