人类淋巴细胞中钙激活钾通道的电压门控

Voltage gating of Ca2(+)-activated potassium channels in human lymphocytes.

作者信息

Mátyus L, Pieri C, Recchioni R, Moroni F, Bene L, Trón L, Damjanovich S

机构信息

Department of Biophysics, University Medical School of Debrecen, Hungary.

出版信息

Biochem Biophys Res Commun. 1990 Aug 31;171(1):325-9. doi: 10.1016/0006-291x(90)91396-a.

DOI:10.1016/0006-291x(90)91396-a

PMID:2393396

Abstract

The effect of membrane potential on Ca2+ activated K+ channels was studied on human peripheral lymphocytes. Membrane potential was monitored using bisoxonol and flow cytometry. 1 mM Ca2+ in the presence of 2 microM ionomycin depolarized the control cell population, while 100 microM Ca2+ caused hyperpolarization. However 1 mM Ca2+ had a hyperpolarizing effect on previously partially depolarized cells. Potassium channel blockers did not influence the depolarization, while they inhibited the hyperpolarization. Based on the experimental evidence a voltage gating of Ca2+ activated K+ channels is suggested.

摘要

在人外周血淋巴细胞上研究了膜电位对钙离子激活钾通道的影响。使用双羟萘酸和流式细胞术监测膜电位。在存在2微摩尔离子霉素的情况下，1毫摩尔钙离子使对照细胞群体去极化，而100微摩尔钙离子则导致超极化。然而，1毫摩尔钙离子对先前部分去极化的细胞具有超极化作用。钾通道阻滞剂不影响去极化，但能抑制超极化。基于实验证据，提示钙离子激活钾通道存在电压门控。