Polat Beyzagul, Albayrak Abdulmecit, Halici Zekai, Karakus Emre, Bayir Yasin, Demirci Elif, Cadirci Elif, Odaci Ersan, Yayla Muhammed, Atamanalp Sabri Selcuk
1 Department of Pharmacology, School of Pharmacy, Ataturk University, Erzurum, Turkey.
J Invest Surg. 2013 Dec;26(6):325-33. doi: 10.3109/08941939.2013.806615. Epub 2013 Aug 19.
Intestinal ischemia reperfusion (IR) is associated with morbidity and mortality. We first examined the role of levosimendan in the protection of intestine after mesenteric IR.
The rats were divided into six groups: (1) Control group; (2) Levosimendan group; (3) Ischemia group (60 min of occlusion); (4) IR group (60 min of occlusion and then 60 min reperfusion); (5) IR + 1 mg/kg levosimendan group: Levosimendan was given intraperitonally 30 min prior to the ischemia; (6) IR + 2 mg/kg levosimendan group.
The levels of TNF-α, IL-6, and IL-1β were found to have increased in the IR group. The serum levels of TNF-α, IL-6, and IL-1β were found to have decreased as a result of the administration of both doses of levosimendan in the IR. Relative TNF-α and NFκB mRNA levels was decreased by administration of both doses of levosimendan in the IR. SOD activity and GSH levels for IR group were lower than, and 8-ISO levels were higher than, those of the sham-operated rat and ischemia alone group.
Both doses of levosimendan had preventive effects on the alterations that occurred in the intestinal tissues after IR. Levosimendan administration attenuated in reperfusion injury of intestine and consequently protects intestinal mucosa and oxidant-antioxidant balance of ileum.
肠道缺血再灌注（IR）与发病率和死亡率相关。我们首先研究了左西孟旦在肠系膜IR后对肠道的保护作用。
将大鼠分为六组：（1）对照组；（2）左西孟旦组；（3）缺血组（阻断60分钟）；（4）IR组（阻断60分钟，然后再灌注60分钟）；（5）IR + 1mg/kg左西孟旦组：在缺血前30分钟腹腔注射左西孟旦；（6）IR + 2mg/kg左西孟旦组。
发现IR组中TNF-α、IL-6和IL-1β水平升高。在IR中给予两种剂量的左西孟旦后，血清TNF-α、IL-6和IL-1β水平降低。在IR中给予两种剂量的左西孟旦后，相对TNF-α和NFκB mRNA水平降低。IR组的SOD活性和GSH水平低于假手术大鼠和单纯缺血组，而8-ISO水平高于它们。
两种剂量的左西孟旦对IR后肠道组织发生的改变均有预防作用。给予左西孟旦可减轻肠道再灌注损伤，从而保护肠黏膜和回肠的氧化-抗氧化平衡。
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