[1,25-二羟维生素D3对ROS 17/2.8成骨细胞形态及碱性磷酸酶活性的影响：时间和剂量的作用]
[Effects of 1,25 dihydroxyvitamin d3 on the morphology and alkaline phosphatase activity of ROS 17/2,8 osteoblastic cells: influence of time and dose].
作者信息
Chavassieux P, Boivin G, Terrier C, Serre C M, Lagarde D, Meunier P J
机构信息
Faculté Alexis Carrel, INSERM Unité 234, Lyon, France.
出版信息
Reprod Nutr Dev. 1990;30(3):439-43.
Osteosarcoma-derived osteoblastic cells were exposed to 1,25 dihydroxyvitamin D3 (1,25(OH)2D3) either to 0.001-1,000 nmol.l-1 for 4 days or to 10 nmol.-1 for 1-21 days. Between 0.01 and 10 nmol.l-1, a dose-dependent increase in alkaline phosphatase (AP) activity was found, which rose to a maximum level at 10 nmol.l-1 (+55%). At higher doses (100 and 1,000 nmol.l-1), 1,25(OH)2D3 induced a decrease in AP activity of 40%. After 1 day at 10 nmol.l-1 a slight increase in AP activity was noted (+20%) which augmented with the duration of exposure. This stimulatory effect was highest after 8 days (+130%). In contrast, after 15 and 21 days, AP activity decreased by 30%. Under 1,25(OH)2D3, microtubules were observed mainly in the perinuclear region.
骨肉瘤来源的成骨细胞分别暴露于浓度为0.001 - 1,000 nmol·l⁻¹的1,25 - 二羟基维生素D3（1,25(OH)₂D3）中4天，或暴露于浓度为10 nmol·l⁻¹的1,25(OH)₂D3中1 - 21天。在0.01至10 nmol·l⁻¹之间，发现碱性磷酸酶（AP）活性呈剂量依赖性增加，在10 nmol·l⁻¹时升至最高水平（增加55%）。在更高剂量（100和1,000 nmol·l⁻¹）下，1,25(OH)₂D3使AP活性降低40%。在10 nmol·l⁻¹下处理1天后，AP活性略有增加（增加20%），且随着暴露时间延长而增强。这种刺激作用在8天后最高（增加130%）。相反，在15天和21天后，AP活性降低了30%。在1,25(OH)₂D3作用下，微管主要出现在核周区域。
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