Department of Breast Surgery, The Third Affiliated Hospital of Harbin Medical University, Harbin, China ; Department of Immunology & Heilongjiang Provincial Key Laboratory for Infection and Immunity, Harbin Medical University, Harbin, China.
PLoS One. 2013 Aug 16;8(8):e71040. doi: 10.1371/journal.pone.0071040. eCollection 2013.
As a costimulatory molecule, Herpesvirus entry mediator (HVEM) can bind with several costimulatory members, thus HVEM plays different roles in T cell immunity. HVEM and its ligands have been involved in the pathogenesis of various autoimmune, inflammatory diseases and tumors. In the current study, we conducted a case-control study comparing polymorphisms of HVEM and breast cancer. Subjects included 575 females with breast cancer and 604 age-matched healthy controls. Six HVEM SNPs (rs2281852, rs1886730, rs2234163, rs11573979, rs2234165, and rs2234167) were genotyped by PCR-RFLP. The results showed significant differences in genotypes and alleles between rs1886730 and rs2234167 (P<0.05). One haplotype (CTGCGG) that was associated with breast cancer was found via haplotype analysis. Our research also indicated an association between polymorphisms of HVEM and clinicopathologic features, including lymph node metastasis, estrogen receptor, progesterone receptor and P53. Our results primarily indicate that polymorphisms of the HVEM gene were associated with the risk of sporadic breast cancer in northeast Chinese females.
作为一种共刺激分子，疱疹病毒进入介体（HVEM）可以与几种共刺激成员结合，因此 HVEM 在 T 细胞免疫中发挥不同的作用。HVEM 及其配体已参与多种自身免疫、炎症性疾病和肿瘤的发病机制。在本研究中，我们进行了一项病例对照研究，比较了 HVEM 和乳腺癌的多态性。研究对象包括 575 名女性乳腺癌患者和 604 名年龄匹配的健康对照者。通过 PCR-RFLP 对 6 个 HVEM SNP（rs2281852、rs1886730、rs2234163、rs11573979、rs2234165 和 rs2234167）进行了基因分型。结果显示 rs1886730 和 rs2234167 的基因型和等位基因存在显著差异（P<0.05）。通过单体型分析发现一个与乳腺癌相关的单体型（CTGCGG）。我们的研究还表明 HVEM 多态性与临床病理特征之间存在关联，包括淋巴结转移、雌激素受体、孕激素受体和 P53。我们的研究结果主要表明 HVEM 基因多态性与中国东北女性散发性乳腺癌的风险相关。
