Yun Yeo Hong, Hyun Min Woo, Suh Dong Yeon, Kim Yong Min, Kim Seong Hwan
Department of Microbiology, Dankook University, Cheonan 330-714, Korea.
Mycobiology. 2009 Dec;37(4):251-7. doi: 10.4489/MYCO.2009.37.4.251. Epub 2009 Dec 31.
We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25℃. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20℃, 37 mm at 25℃ and 32 mm at 30℃ after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25℃) compared to CYA and PDA (< 40 mm 15 days at 25℃). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 4075 µm in diameter. Asci are 8-spored and globose to subglobose 811 µm. Ascospores are disciform, 4.05.0 µm in length and 4.24.5 µm in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.66.0 µm in length and 3.04.3 µm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in β-tubulin (1016 bp) gene. The E. rubrum isolate also had weak β-glucosidase and protease activities.
我们从韩国尚未报道过的麴中分离并鉴定出一株红曲霉。这种真菌呈黄棕色；在CYA和PDA培养基上，其背面为深棕色，而在25℃的2% MEA培养基上为黄色。闭囊壳最初为亮黄色，逐渐变为棕色。在CYA培养基上，15天后，菌丝体在20℃时直径达到30 mm，25℃时达到37 mm，30℃时达到32 mm。与CYA和PDA培养基相比（25℃下15天<40 mm），该分离株在2% MEA培养基上生长较慢（25℃下15天<20 mm）。闭囊壳表面生，黄色至浅棕色，球形至近球形，直径4075 µm。子囊有8个孢子，球形至近球形，811 µm。子囊孢子呈盘状，长4.05.0 µm，宽4.24.5 µm。分生孢子卵形或杆状，表面微粗糙至密被小刺，长4.66.0 µm，宽3.04.3 µm。与已知的红曲霉相比，韩国分离株在ITS rDNA（554 bp）和钙调蛋白（750 bp）基因上显示出99%的序列相似性，在β-微管蛋白（1016 bp）基因上显示出100%的序列相似性。该红曲霉分离株还具有较弱的β-葡萄糖苷酶和蛋白酶活性。
