响尾蛇毒素酸性亚基B链的氨基酸序列。

The amino acid sequence of the acidic subunit B-chain of crotoxin.

作者信息

Aird S D, Yates J R, Martino P A, Shabanowitz J, Hunt D F, Kaiser I I

机构信息

Department of Molecular Biology, University of Wyoming, Laramie 82071.

出版信息

Biochim Biophys Acta. 1990 Sep 3;1040(2):217-24. doi: 10.1016/0167-4838(90)90079-u.

DOI:10.1016/0167-4838(90)90079-u

PMID:2400773

Abstract

The B-chain of the acidic subunit of crotoxin proved refractory to Edman degradation. When subjected to sequence analysis using tandem mass spectrometry, pyroglutamate was found at the amino-terminal end, even though earlier attempts to de-block with pyroglutamate aminopeptidase were unsuccessful. The B-chain contained 35 amino acids and showed 91% amino acid identity with the corresponding segment from Mojave toxin, a homologous neurotoxin from Crotalus scutulatus scutulatus. The sequence of the last 24 residues of the B-chain is consistent with that previously published (Aird, S.D., Kaiser, I.I., Lewis, R.V. and Kruggel, W.G. (1985) Biochemistry 24, 7054-7058), except at position 20, where Edman degradation gave glycine and mass spectrometry gave glutamic acid.

摘要

响尾蛇毒素酸性亚基的B链难以用埃德曼降解法进行测序。当使用串联质谱进行序列分析时，尽管早期用焦谷氨酸氨肽酶去封闭的尝试未成功，但在氨基末端发现了焦谷氨酸。B链含有35个氨基酸，与莫哈韦毒素（一种来自角响尾蛇的同源神经毒素）的相应片段有91%的氨基酸同一性。B链最后24个残基的序列与先前发表的序列一致（Aird, S.D., Kaiser, I.I., Lewis, R.V. 和 Kruggel, W.G. (1985) Biochemistry 24, 7054 - 7058），除了第20位，埃德曼降解法得到的是甘氨酸，而质谱法得到的是谷氨酸。