[利用纤维素结合结构域融合标签在谷氨酸棒杆菌中高效生产蛋白质]
[Cost-effective production of protein by using cellulose-binding domain fusion tag in Corynebacterium glutamicum].
作者信息
Zhao Zhijing, Jiang Huan, Shen Wenting, Song Lianyan, Hu Guang
机构信息
Bioengineering Center, Shaoxing Institute of Technology, College of Engineering, Peking University, Shaoxing 312000, Zhejiang, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2013 May;29(5):691-4.
The CBD gene from Trichoderma reesei was cloned into the Corynebacterium glutamicum secretion expression vector pXMJ19-sp, in which green fluorescent protein was inserted to obtain pXMJ19-sp-GFP-CBD. After induced by 0.5 mmol/L IPTG, GFP-CBD was expressed in Corynebacterium glutamicum at high level of 200 mg/L. The GFP-CBD could be purified to high purity with cellulose column. The results indicated CBD can be successfully used in Corynebacterium glutamicum expression system and thus offer an extremely simple, effective and scalable way for production of recombinant proteins.
将里氏木霉的CBD基因克隆到谷氨酸棒杆菌分泌表达载体pXMJ19-sp中，该载体插入了绿色荧光蛋白，从而获得pXMJ19-sp-GFP-CBD。经0.5 mmol/L IPTG诱导后，GFP-CBD在谷氨酸棒杆菌中高水平表达，达到200 mg/L。GFP-CBD可用纤维素柱纯化至高纯度。结果表明，CBD可成功用于谷氨酸棒杆菌表达系统，从而为重组蛋白的生产提供了一种极其简单、有效且可扩展的方法。
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