Department of Periodontology, School of Dentistry, Ege University, İzmir, Turkey.
Arch Oral Biol. 2013 Oct;58(10):1302-8. doi: 10.1016/j.archoralbio.2013.07.010. Epub 2013 Aug 15.
This study was performed to evaluate gingival crevicular fluid (GCF) and serum levels of a proliferation inducing ligand (APRIL) and B cell activating factor (BAFF) and compare this to differences between TNF-alpha levels in rheumatoid arthritis (RA), osteoporosis (OPR) and systemically healthy women with periodontal disease (SH).
Gingival crevicular fluid (GCF) and serum samples were obtained before any periodontal intervention from 17 RA, 19 OPR patients and 13 SH women with periodontitis. Full-mouth clinical periodontal measurements were recorded. APRIL, BAFF and TNF-α levels were determined by ELISA. Statistical analysis was performed using multivariate analysis, ANOVA and Spearman correlation.
Pocket depths differed in site-specific comparisons, but otherwise clinical measurements were similar in the three study groups. Multivariate least squares regression ANOVA adjusted for age and for plaque index indicated that total amounts of TNF-α and concentrations of TNF-α, BAFF and APRIL were significantly greater in the RA patients than in the SH group (p<0.05), and GCF concentrations of BAFF were greater in OPR patients than in SH. Serum TNF-α and BAFF were significantly higher in the RA group compared to SH (p<0.05) and serum TNF-α was greater in RA than in OPR (p<0.05). APRIL and BAFF correlated with RANKL levels in GCF and serum (p<0.05).
Despite long-term usage of anti-inflammatory drugs in the RA and OPR patients, increased TNF-family cytokines, might suggest that these patients have a propensity to overproduce these inflammatory mediators but whether this results from greater disease activity or contribute to greater disease activity remains moot.
本研究旨在评估牙龈沟液（GCF）和血清中增殖诱导配体（APRIL）和 B 细胞激活因子（BAFF）的水平，并将其与类风湿关节炎（RA）、骨质疏松症（OPR）和牙周病系统健康女性（SH）的 TNF-α 水平差异进行比较。
在任何牙周干预之前，从 17 例 RA、19 例 OPR 患者和 13 例 SH 牙周炎女性中采集龈沟液（GCF）和血清样本。记录全口临床牙周测量值。通过 ELISA 测定 APRIL、BAFF 和 TNF-α 水平。采用多元分析、方差分析和 Spearman 相关分析进行统计分析。
在特定部位的比较中，牙周袋深度存在差异，但在这三个研究组中，临床测量值相似。多元最小二乘回归方差分析调整年龄和菌斑指数表明，RA 患者的 TNF-α 总量以及 TNF-α、BAFF 和 APRIL 的浓度明显高于 SH 组（p<0.05），OPR 患者的 GCF 中 BAFF 浓度高于 SH 组。与 SH 相比，RA 组血清 TNF-α 和 BAFF 明显升高（p<0.05），RA 组血清 TNF-α 高于 OPR 组（p<0.05）。GCF 和血清中的 APRIL 和 BAFF 与 RANKL 水平呈正相关（p<0.05）。
尽管 RA 和 OPR 患者长期使用抗炎药物，但 TNF 家族细胞因子的增加可能表明这些患者倾向于过度产生这些炎症介质，但这是否是由于疾病活动度增加所致，或者是否会导致疾病活动度增加，仍存在争议。
