信息素诱导的小鼠犁鼻器感觉系统中即早基因的表达。

Pheromone-induced expression of immediate early genes in the mouse vomeronasal sensory system.

作者信息

Haga-Yamanaka Sachiko, Touhara Kazushige

机构信息

Stowers Institute for Medical Research, Kansas City, MO, USA.

出版信息

Methods Mol Biol. 2013;1068:247-58. doi: 10.1007/978-1-62703-619-1_18.

DOI:10.1007/978-1-62703-619-1_18

PMID:24014367

Abstract

Immediate early genes (IEGs) are powerful tools for visualizing activated neurons and extended circuits that are stimulated by sensory input. Several kinds of IEGs (e.g., c-fos, egr-1) have been utilized for detecting activated receptor neurons in the pheromone sensory organ called the vomeronasal organ (VNO), as well as for mapping the neurons within the central nervous system (CNS) excited by pheromones.In this chapter, we describe the procedure for the detection of pheromone-induced neural activation in the VNO and CNS using the c-Fos immunostaining technique.

摘要

即刻早期基因（IEGs）是用于可视化由感觉输入刺激的激活神经元和扩展回路的强大工具。几种即刻早期基因（例如，c-fos、egr-1）已被用于检测在称为犁鼻器（VNO）的信息素感觉器官中激活的受体神经元，以及绘制由信息素激发的中枢神经系统（CNS）内的神经元图谱。在本章中，我们描述了使用c-Fos免疫染色技术检测犁鼻器和中枢神经系统中信息素诱导的神经激活的程序。