神经纤毛蛋白-1 的细胞质结构域调节粘着斑的转化。
The cytoplasmic domain of neuropilin-1 regulates focal adhesion turnover.
机构信息
Department of Molecular Cardiology, Lerner Research Institute, the Cleveland Clinic, Cleveland, OH 44195, United States.
出版信息
FEBS Lett. 2013 Nov 1;587(21):3392-9. doi: 10.1016/j.febslet.2013.08.040. Epub 2013 Sep 8.
Abstract
Though the vascular endothelial growth factor coreceptor neuropilin-1 (Nrp1) plays a critical role in vascular development, its precise function is not fully understood. We identified a group of novel binding partners of the cytoplasmic domain of Nrp1 that includes the focal adhesion regulator, Filamin A (FlnA). Endothelial cells (ECs) expressing a Nrp1 mutant devoid of the cytoplasmic domain (nrp1(cyto)(Δ/Δ)) migrated significantly slower in response to VEGF relative to the cells expressing wild-type Nrp1 (nrp1(+/+) cells). The rate of FA turnover in VEGF-treated nrp1(cyto)(Δ/Δ) ECs was an order of magnitude lower in comparison to nrp1(+/+) ECs, thus accounting for the slower migration rate of the nrp1(cyto)(Δ/Δ) ECs.
摘要
虽然血管内皮生长因子核心受体神经纤毛蛋白-1（Nrp1）在血管发育中起着关键作用，但它的确切功能尚不完全清楚。我们鉴定了一组 Nrp1 胞质域的新结合伴侣，其中包括黏着斑调节因子细丝蛋白 A（FlnA）。与表达野生型 Nrp1（nrp1(+/+) 细胞）的细胞相比，表达无胞质域 Nrp1 突变体（nrp1(cyto)(Δ/Δ)）的内皮细胞（EC）对 VEGF 的迁移速度明显较慢。与 nrp1(+/+) EC 相比，VEGF 处理的 nrp1(cyto)(Δ/Δ) EC 中的 FA 周转率低一个数量级，这解释了 nrp1(cyto)(Δ/Δ) EC 迁移速度较慢的原因。
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2
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Cell Mol Life Sci. 2012 Nov;69(22):3765-805. doi: 10.1007/s00018-012-1019-0. Epub 2012 Jun 29.
3
Regulation of VEGF signaling by membrane traffic.
Cell Signal. 2012 Sep;24(9):1810-20. doi: 10.1016/j.cellsig.2012.05.007. Epub 2012 May 19.
4
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J Cell Sci. 2012 Jan 15;125(Pt 2):497-506. doi: 10.1242/jcs.094433. Epub 2012 Feb 2.
5
The structure of cell-matrix adhesions: the new frontier.
Curr Opin Cell Biol. 2012 Feb;24(1):134-40. doi: 10.1016/j.ceb.2011.12.001. Epub 2011 Dec 22.
6
Structure and function of focal adhesions.
Curr Opin Cell Biol. 2012 Feb;24(1):116-24. doi: 10.1016/j.ceb.2011.11.001. Epub 2011 Dec 2.
7
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8
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9
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10
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