Quan Xiang-Chun, Cen Yan, Qian Yin
School of Environment, Beijing Normal University, Beijing 100875, China.
Huan Jing Ke Xue. 2013 Jul;34(7):2862-8.
Two aerobic denitrifying bacteria were isolated from the sludge of municipal wastewater treatment plant by enrichment, preliminary screening with BTB culture medium and the denitrification potential test and identified through 16S rDNA sequence analysis. Then they were bioaugmented to oligotrophic biofilm system respectively, aiming to enhance the denitrification capacity. The two strains were identified as Pseudomonas aeruginosa and Pseudomonas putida respectively. They could remove 78% or 82% of the total nitrogen in the simulate wastewater when existed alone. And the total nitrogen removal efficiency of the biofilm system reached 68% and 64% after bioaugmentation with two trains, increased by 47% and 43% compared to the control, respectively. Meanwhile, the ammonia nitrogen was nearly 100% removed. It can be concluded that two aerobic denitrifiers can enhance the denitrification of biofilm system significantly under oligotrophic conditions and will not inhibit the nitrification process, therefore can help biofilm system achieve simultaneous nitrification and denitrification.
通过富集培养、使用溴百里酚蓝（BTB）培养基进行初步筛选以及反硝化潜力测试，从城市污水处理厂的污泥中分离出两株好氧反硝化细菌，并通过16S rDNA序列分析对其进行鉴定。然后将它们分别添加到贫营养生物膜系统中，以提高反硝化能力。这两株菌分别被鉴定为铜绿假单胞菌和恶臭假单胞菌。它们单独存在时能够去除模拟废水中78%或82%的总氮。用这两株菌进行强化后，生物膜系统的总氮去除效率分别达到68%和64%，与对照相比分别提高了47%和43%。同时，氨氮几乎被100%去除。可以得出结论，两株好氧反硝化菌在贫营养条件下能显著提高生物膜系统的反硝化能力，且不会抑制硝化过程，因此有助于生物膜系统实现同步硝化反硝化。
