Song Zhaoqi, Wang Li, Chen Jinquan, Zhou Enmin, Zhang Chuanlun, Li Wenjun
Engineering Technology Research Center of Biomass Degradation and Gasification, University of Henan Province, Shangqiu Normal University, Shangqiu 476000, China.
Wei Sheng Wu Xue Bao. 2013 Jun 4;53(6):569-76.
Crenarchaeota is a major archaeal lineage in terrestrial hot springs and important in biogeochemical cycles of life-essential elements. In this study, we investigated the diversity of Crenarchaeota in hot springs and the surrounding environments in Kamchatka, Russia. In addition, we compared crenarchaeotal community structures in Kamchatka, Russia and Yunnan province, China.
Crenarchaeal 16S rRNA gene clone libraries were constructed and the sequences and abundances of representational clone were obtained. Phylogenetic analysis was then performed and the community structures in different samples were compared.
The high temperature spring Burlyashi Liza (BSL, 89 degrees C) comprised Thermoprotei. The moderate temperature spring TF Vent 2 (TFV, 49 degrees C) harbored unidentified Thermoprotei group, unidentified crenarchaeal group, HWCG-II (hot water crenarchaeotal group II), and Group1. 1b (one thaumarchaeotal subgroup). Most of sequences that obtained from surrounding environments ( <15 degrees C) are closely with the representational clone pJP from a Yellowstone hot spring. Jackknife cluster and Principal coordinates analysis (PCoA) showed that the samples have more similarity in crenarchaeal communities at similar temperatures.
The diversities of Crenarchaeota in Kamchatka hot springs are somewhat different from those in Yunnan province. Terrestrial hot springs obviously affect the crenarchaeotal communities in surrounding environments. Temperature is the major factor controlling the community structure in terrestrial hot springs.
泉古菌是陆地温泉中主要的古菌谱系，在生命必需元素的生物地球化学循环中具有重要作用。在本研究中，我们调查了俄罗斯堪察加半岛温泉及其周边环境中泉古菌的多样性。此外，我们还比较了俄罗斯堪察加半岛和中国云南省泉古菌的群落结构。
构建泉古菌16S rRNA基因克隆文库，获得代表性克隆的序列和丰度。然后进行系统发育分析，并比较不同样本中的群落结构。
高温泉布尔利亚希利扎（BSL，89摄氏度）包含热变形菌纲。中温泉TF Vent 2（TFV，49摄氏度）含有未鉴定的热变形菌群、未鉴定的泉古菌群、HWCG-II（热水泉古菌群II）和Group1. 1b（一个奇古菌亚群）。从周边环境（<15摄氏度）获得的大多数序列与黄石温泉的代表性克隆pJP密切相关。刀切聚类和主坐标分析（PCoA）表明，相似温度下的样本在泉古菌群落方面具有更高的相似性。
堪察加半岛温泉中泉古菌的多样性与云南省的有所不同。陆地温泉明显影响周边环境中的泉古菌群落。温度是控制陆地温泉群落结构的主要因素。
