虹鳟鱼肝细胞色素P450同工酶对月桂酸羟基化的区域特异性
Regiospecificity in the hydroxylation of lauric acid by rainbow trout hepatic cytochrome P450 isozymes.
作者信息
Miranda C L, Wang J L, Henderson M C, Williams D E, Buhler D R
机构信息
Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.
出版信息
Biochem Biophys Res Commun. 1990 Sep 14;171(2):537-42. doi: 10.1016/0006-291x(90)91179-v.
The catalytic activity of two hepatic cytochrome P450 isozymes from untreated rainbow trout towards lauric acid was investigated. In a reconstituted system, cytochrome P450 LMC1 and P450 LMC2 were found to catalyze exclusively the omega- and (omega-1)-hydroxylation of lauric acid, respectively. Microsomal enzyme inhibition studies with polyclonal antibodies raised against the individual P450 isozymes showed that P450 LMC1 and LMC2, respectively, accounted for most if not all the omega- and (omega-1)-lauric acid hydroxylase activity of trout liver microsomes. The polyclonal antibodies were highly specific in that they only inhibited the enzyme activity of the P450 used as the immunogen. These results illustrate that as in mammals, omega- and (omega-1)-hydroxylation of lauric acid by trout liver microsomes can be carried out separately by distinct isozymes of cytochrome P450.
研究了未经处理的虹鳟鱼两种肝细胞色素P450同工酶对月桂酸的催化活性。在重组系统中，发现细胞色素P450 LMC1和P450 LMC2分别只催化月桂酸的ω-和（ω-1）-羟基化反应。用针对单个P450同工酶产生的多克隆抗体进行的微粒体酶抑制研究表明，P450 LMC1和LMC2分别占虹鳟鱼肝微粒体中大部分（如果不是全部）的ω-和（ω-1）-月桂酸羟化酶活性。多克隆抗体具有高度特异性，因为它们只抑制用作免疫原的P450的酶活性。这些结果表明，与哺乳动物一样，虹鳟鱼肝微粒体对月桂酸的ω-和（ω-1）-羟基化反应可由细胞色素P450的不同同工酶分别进行。
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