Kasperbauer J L, Neel H B, Scott R E
Section of Experimental Pathology, Mayo Clinic/Foundation, Rochester, Minnesota.
Ann Otol Rhinol Laryngol. 1990 Jan;99(1):29-37. doi: 10.1177/000348949009900105.
Normal human squamous mucosal cells of the soft palate, buccal surface, epiglottis, hypopharynx, floor of the mouth, and tongue were cultured in vitro in serum-free medium. In medium MCDB 153 containing epidermal growth factor, insulin, bovine pituitary extract, and 0.1 or 2.0 mmol/L Ca++, squamous mucosal cells double every 24 hours. These cells then can be induced to arrest their proliferation reversibly by treatment with transforming growth factor-beta or ethionine, and they can irreversibly growth-arrest during senescence or when cultured in growth factor-deficient medium containing 2 mmol/L Ca++. The latter medium also induces differentiation, as does culture of cells in serum-containing medium. Serum-containing medium furthermore promotes extensive cell stratification and the formation of multilayered squamous mucosal tissue specimens that can be removed intact by Dispase treatment. These specimens represent potential autogenous mucosal grafts that can be used in patients who require reconstructive surgery of the oral cavity and oropharynx. Normal human squamous mucosal cells therefore closely resemble keratinocytes derived from the epidermis in the mechanisms that regulate proliferation and differentiation. This model cell system should facilitate future studies on upper aerodigestive tract squamous mucosal cell physiology and pathophysiology.
将正常人软腭、颊面、会厌、下咽、口底和舌的鳞状黏膜细胞在无血清培养基中进行体外培养。在含有表皮生长因子、胰岛素、牛垂体提取物和0.1或2.0 mmol/L钙离子的MCDB 153培养基中，鳞状黏膜细胞每24小时倍增一次。然后，通过用转化生长因子-β或乙硫氨酸处理，这些细胞可被诱导可逆地停止增殖，并且在衰老期间或在含有2 mmol/L钙离子的生长因子缺乏培养基中培养时，它们会不可逆地生长停滞。后一种培养基还可诱导分化，含血清培养基培养细胞时也会如此。含血清培养基还能促进广泛的细胞分层以及形成多层鳞状黏膜组织标本，这些标本可通过Dispase处理完整移除。这些标本代表了潜在的自体黏膜移植物，可用于需要口腔和口咽重建手术的患者。因此，正常人鳞状黏膜细胞在调节增殖和分化的机制上与源自表皮的角质形成细胞非常相似。这种模型细胞系统应有助于未来对上呼吸道消化道鳞状黏膜细胞生理学和病理生理学的研究。
