草绿龙蜥（有鳞目，蜥蜴科）的完整线粒体基因组
Complete mitochondrial genome of Takydromus sexlineatus (Squamata, Lacertidae).
作者信息
Qin Ping-Sheng, Zeng De-Long, Hou Li-Xia, Yang Xiao-Wen, Qin Xin-Min
机构信息
Guangxi Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University , Guilin, Guangxi , P.R. China.
出版信息
Mitochondrial DNA. 2015 Jun;26(3):465-6. doi: 10.3109/19401736.2013.830299. Epub 2013 Sep 19.
The complete sequence mitochondrial genome of Takydromus sexlineatus was determined using long PCR and conserved primers walking approaches. The genome was 18,943 bp in length and contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 1 control region (CR). The gene composition and order of T. sexlineatus were similar to most other squamate reptiles. All protein-coding genes begin with ATG as initiation codon except COI using GTG. Seven genes (ATP8. ND4L. ND5. Cytb. ND1. COI and ND6) ended with TAA, TAG, AGGA and AGA stop codon, the remaining 6 genes had incomplete stop codons T/TA. The overall base composition of the genome in descending order was 31.48% A, 24.67% C, 30.79% T and 13.05% G, with a slight A + T bias of 62.27%. CR is located between the tRNA-Pro and tRNA-Phe genes and is 3562 bp in length, some tandem repeat sequences, conserved elements (CSB1-3) and termination associated sequences (TAS1-3) were found in the control region.
利用长片段PCR和保守引物步移法测定了草绿龙蜥的线粒体基因组全序列。该基因组长度为18,943 bp，包含13个蛋白质编码基因、2个rRNA基因、22个tRNA基因和1个控制区（CR）。草绿龙蜥的基因组成和顺序与大多数其他有鳞目爬行动物相似。除COI基因以GTG作为起始密码子外，所有蛋白质编码基因均以ATG作为起始密码子。7个基因（ATP8、ND4L、ND5、Cytb、ND1、COI和ND6）以TAA、TAG、AGGA和AGA作为终止密码子，其余6个基因具有不完整的终止密码子T/TA。基因组的总体碱基组成从高到低依次为31.48%的A、24.67%的C、30.79%的T和13.05%的G，A+T含量略偏高，为62.27%。控制区位于tRNA-Pro和tRNA-Phe基因之间，长度为3562 bp，在控制区发现了一些串联重复序列、保守元件（CSB1-3）和终止相关序列（TAS1-3）。
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