用于组蛋白乙酰化活细胞成像的基因编码荧光共振能量转移指示剂。

Genetically encoded FRET indicators for live-cell imaging of histone acetylation.

作者信息

Sasaki Kazuki, Yoshida Minoru

机构信息

Chemical Genetics Laboratory/Chemical Genomics Research Group, RIKEN Advanced Science Institute, Wako, Japan.

出版信息

Methods Mol Biol. 2014;1071:151-61. doi: 10.1007/978-1-62703-622-1_12.

DOI:10.1007/978-1-62703-622-1_12

PMID:24052387

Abstract

Histone acetylation is dynamically and reversibly controlled by histone acetyltransferases and deacetylases during cellular events such as cell division and differentiation. However, the dynamics of histone modifications in living cells are poorly understood because of the lack of experimental tools to monitor them in a real-time fashion. Herein, we introduce Förster/fluorescence resonance energy transfer (FRET)-based indicators to visualize acetylation of histone H4, and describe a protocol for live-cell imaging with high spatiotemporal resolution.

摘要

在细胞分裂和分化等细胞活动过程中，组蛋白乙酰化由组蛋白乙酰转移酶和去乙酰化酶动态且可逆地控制。然而，由于缺乏实时监测活细胞中组蛋白修饰动态变化的实验工具，人们对其了解甚少。在此，我们引入基于Förster/荧光共振能量转移（FRET）的指示剂来可视化组蛋白H4的乙酰化，并描述一种具有高时空分辨率的活细胞成像方案。